oriTDB
The information of the oriT region
oriTDB accession number 100117
Name oriT_pSF301-3  insolico
Sequence Completeness core
oriT length 33 nt
oriT sequence GTAGCGATAGCGGAGTGTATACTGGCTTAACCA
IR (inverted repeat)_
Location of nic site [25-26] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note ColE1 superfamily
D:25-26
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 112
Name Mob_pSF301-3 insolico
GenBank accession number YP_006961080
Family MOBP
Length 537 aa
UniProt ID F6MFW0
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.00E-20, Aligned region: 65..262
Note Relaxase; plasmid mobilization protein
Protein sequence [Download] MSCAGCVWPSGNRGRGMIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
DATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLP
ETKQALAESITRWIDALYHVGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIR
LKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARRICKQGCDIKRDENQRRYSPV
HSFDRGITEKTSGRGERGDDAAQEGRVKAGREYGHDVTGDGHFPVYREWRDALVSWRDDT
GEPGRNQEAERESGEAEREDMGRALSRGEQRTFSGVARGDESRNELDDGKRETECRKAGT
GVTEHDGAGKPFAERIRAAATGLYAAAERMGERLRGIAENVFAYATGQRDAERAGHAVES
AGAALERADRTLEPVIQRELEIREERLIQEREHALFLERERQPEIQERTLDGPSLGW
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 168 _ insolico
 N/A
Information of plasmid
ID 112
Plasmid name pSF301-3
GenBank accession number NC_019250.1
Genome size 4043 bp
Coordinate of oriT  [Strand] 228..260 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Shigella flexneri 2a 301 [623]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU