oriTDB

The information of the oriT region

oriTDB accession number	100116
Name	oriT_pSC101
Sequence Completeness	core
oriT length	45 nt
oriT sequence	GGGCGCACGTTTCTGAACGAAGTGAAGAAAGTCTAAGTGCGCCCT
IR (inverted repeat)	[10-18] [22-30] nt (TTTCTGAAC.. GTGAAGAAA)
Location of nic site	[41-42] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	IncQ type mobilizable plasmid
	L:10-18;R:22-30;D:41-42

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGCGCACGTTTCTGAACGAAGTGAAGAAAGTCTAAGTGCGCCCT

Reference

[1] Meyer R (2000) Identification of the mob genes of plasmid pSC101 and characterization of a hybrid pSC101-R1162 system for conjugal mobilization. J Bacteriol. 182(17):4875-81. [PMID:10940031]

[2] Jandle S et al (2006) Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer. J Bacteriol. 188(2):499-506. [PMID:16385040]

[3] Parker C et al (2005) Elements in the co-evolution of relaxases and their origins of transfer. Plasmid . 53(2):113-8. [PMID:15737398]

I. Information of Relaxase

ID	111
Name	MobA_pSC101
GenBank accession number	NP_044284
Family	MOB_Q
Length	371 aa
UniProt ID	P14492
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 1.10E-30, Aligned region: 17..224
Note	relaxase
Protein sequence [Download]	MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNMPAWAAHKPSEFWKAA DTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHAYQFAIHNPKAAIAGGE QPHAHIMFSERINDGIHRDPEQYFKRANTKEPDAVAQKRHVSGKHRPNAKNTLLPRGRRW ADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQRFDTEQLQAILERREAERQ VQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQESHSGRTFDFEKEPDKLNAL VSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERERLAEKQRQQEKERQRLAEQIR QKPDKGWSFSR

Reference

[1] Meyer R (2000) Identification of the mob genes of plasmid pSC101 and characterization of a hybrid pSC101-R1162 system for conjugal mobilization. J Bacteriol. 182(17):4875-81. [PMID:10940031]

[2] Jandle S et al (2006) Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer. J Bacteriol. 188(2):499-506. [PMID:16385040]

[3] Becker EC et al (2012) Origin and fate of the 3' ends of single-stranded DNA generated by conjugal transfer of plasmid R1162. J Bacteriol. 194(19):5368-76. [PMID:22865840]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	167	MobX_pSC101	NP_044283	194 aa

N/A

Information of plasmid

ID	111
Plasmid name	pSC101
GenBank accession number	NC_002056.1
Incompatibility group	IncQ1
Genome size	9263 bp
Coordinate of oriT [Strand]	4071..4115 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica serovar Typhimurium [90371]

Reference

[1] Tait RC et al (1978) On the nature of tetracycline resistance controlled by the plasmid pSC101. Cell. 13(1):73-81. [PMID:340050]