oriTDB

The information of the oriT region

oriTDB accession number	100115
Name	oriT_pU302S
Sequence Completeness	intact
oriT length	241 nt
oriT sequence	CTCCGTATCTGACATGGGTGTCGGGGCAAAGCCCTGACCAGGGTAATTGTAATAGCGTGC ATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGACA AAAGCACGGCAGTTTTTTGGCAGTGACAGTGTTGTCTGACAGAAATGCCCGTCAAAGAGT CAGACAGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACTCGATATAATCACACAA C
IR (inverted repeat)	IR1: [20-27] [31-38] nt (GTCGGGGC..GCCCTGAC) IR2: [45-62] [65-82] nt (AATTGTAATAGCGTGCAT..ATGCGCGGTATAACAATT) IR3: [150-160] [179-189]nt (TGTTGTCTGAC..GTCAGACAGCA)
Location of nic site	[92-93] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	ColE1 superfamily
	L: 20-27;R:31-38;L:45-62;R:65-82;L:150-160;R:179-189;D:92-93

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTCCGTATCTGACATGGGTGTCGGGGCAAAGCCCTGACCAGGGTAATTGTAATAGCGTGC

61

ATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGACA

121

AAAGCACGGCAGTTTTTTGGCAGTGACAGTGTTGTCTGACAGAAATGCCCGTCAAAGAGT

181

CAGACAGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACTCGATATAATCACACAA

241

C

Reference

[1] Chen CY et al (2007) Complete nucleotide sequences of 84.5- and 3.2-kb plasmids in the multi-antibiotic resistant Salmonella enterica serovar Typhimurium U302 strain G8430. Plasmid. 57(1):29-43. [PMID:16828159]

N/A

Information of plasmid

ID	110
Plasmid name	pU302S
GenBank accession number	NC_006815.1
Genome size	3208 bp
Coordinate of oriT [Strand]	1211..1451 [-]
Drug resistance	resistance to kanamycin (aph(3 )-I gene)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica serovar Typhimurium G8430 [90371]

Reference

[1] Chen CY et al (2007) Complete nucleotide sequences of 84.5- and 3.2-kb plasmids in the multi-antibiotic resistant Salmonella enterica serovar Typhimurium U302 strain G8430. Plasmid. 57(1):29-43. [PMID:16828159]