oriTDB

The information of the oriT region

oriTDB accession number	100113
Name	oriT_pIE321
Sequence Completeness	intact
oriT length	331 nt
oriT sequence	CCTCTCCTGTAGTGTTTCCGTAGTGGTTCAATCCTAGCATTTACAAGGCTTTCCGCCAAT ATTGTAGTAGTCGAACACTACAGCGGTTTTCGTCCTTTGCGTGGAAGTCATTGTAAATCA ATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTAAGGATACCAACC CTGCTTTTAAGGACGAAAACCATGCCGATAACGCCAGCGTGACCCTAAAGAGGGTCAAAA CTGCTCCCAATGCGCTATGCGCATTGGGTTATCGCGCAACAATGATGCACTTATAATGCT ATGATAGTGCTACGATGATGCAGAAAATGAG
IR (inverted repeat)	IR1: [105-112] [120-127] nt (AAGTCATT..AATGACTT) IR2: [131-136] [141-146] nt (CGCACC..GGTGCG) IR3: [246-255] [259-268] nt (CCCAATGCGC..GCGCATTGGG)
Location of nic site	[154-155]
Conserved sequence flanking the nic site	TGTCT\|ATAGC
Note	_
	L:105-112;R:120-127;L:131-136;R:141-146;L:246-255;R:259-268;D:154-155

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCTCTCCTGTAGTGTTTCCGTAGTGGTTCAATCCTAGCATTTACAAGGCTTTCCGCCAAT

61

ATTGTAGTAGTCGAACACTACAGCGGTTTTCGTCCTTTGCGTGGAAGTCATTGTAAATCA

121

ATGACTTACGCGCACCGAAAGGTGCGTATTGTCTATAGCCCAGATTTAAGGATACCAACC

181

CTGCTTTTAAGGACGAAAACCATGCCGATAACGCCAGCGTGACCCTAAAGAGGGTCAAAA

241

CTGCTCCCAATGCGCTATGCGCATTGGGTTATCGCGCAACAATGATGCACTTATAATGCT

301

ATGATAGTGCTACGATGATGCAGAAAATGAG

Reference

[1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]

I. Information of Relaxase

ID	108
Name	TrwC_pIE321
GenBank accession number	YP_001911166
Family	MOB_F
Length	966 aa
UniProt ID	A7KZU5
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.20E-83, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 5.20E-46, Aligned region: 472..659 Viral_helicase1 [PF01443.17], Evalue: 2.80E-06, Aligned region: 492..686 UvrD_C_2 [PF13538.5], Evalue: 3.90E-07, Aligned region: 847..894 AAA_19 [PF13245.5], Evalue: 8.60E-06, Aligned region: 477..601
Note	conjugative relaxase
Protein sequence [Download]	MLSHMVLTRQDIGRAASYYEDGADDYYAKDGDASEWQGKGAEELGLSGEVDSKRFRELLA GNIGEGHRIMRSATRQDSKERIGLDLTFSAPKSVSLQALVAGDAEIIKAHDRAVARTLEQ AEARAQARQKIQGKTRIETTGNLVIGKFRHETSRERDPQLHTHAVILNMTKRSDGQWRAL KNDEIVKATRYLGAVYNAELAHELQKLGYQLRYGKDGNFDLAHIDRQQIEGFSKRTEQIA EWYAARGLDPNSVSLEQKQAAKVLSRAKKTSVDREALRAEWQATAKELGIDFSRREWSGR EKGGSEKQAHSFMPSDEAAKRAVRYAINHLTERQSVMDERELVDTAMKHAVRAARLEDIQ KELLRQTETGYLIREAPRYRPGGQTGPTDEPGKTRAEWVAELAAKGMKQGAARERVDNAI KTGGLVPIEPRYTTQTALEREKRILQIERDGRGAVAPVIAAEVARERLASTNLNQGQREA AELIVSAANRVVGVQGFAGTGKSHMLDTAKQMIEGEGYHVRALAPYGSQVKALRELNVEA NTLASFLRAKDKNIDSRTVLVIDEAGVVPTRLMEQTLKLAEKAGARVVLMGDTAQTKAIE AGRPFDQLQAAGMQTAHMREIQRQKNPELKVAVELAASGKASSSLERIKDVTEIKDHHER RAAVAEAYIALKPDERDRTLIVSGTNEARREINQMVREGLGTAGKGIEFDTLVRVDTTQA ERRHSKNYQVGHVIQPERDYAKTGLQRGELYRVVDTGPGNRLTVVGERDGQRIQFSPMTH TKISVYQPERAELAVGDMVRITRNDKDLDLANGDRMKVVAVEDRKVTVTDGKRNVELPTD KPLHVDHAYATTVHSSQGLTSDRVLIDAHAESRTTAKDVYYVAISRARFEARVFTNDRGK LPAAIARENIKSAAHDLARDRGGRSAAAERQREQQREAERNRQTQQPAHDRQKAAREAER GMEAGR

Reference

[1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	164	TrwA_pIE321	YP_001911164	120 aa	relaxosome accessory protein

Information of T4CP

ID	108
Name	TrwB _pIE321
SecReT4 accesion number	189009837
GenBank accession number	YP_001911165
Family	TrwB/TraD
Length	507
UniProt ID	A7KZU4
PDB ID	_
Pfam	TrwB_AAD_bind [PF10412.8], Evalue: 1.40E-97, Aligned region: 109..491 TraG-D_C [PF12696.6], Evalue: 2.70E-21, Aligned region: 350..472 T4SS-DNA_transf [PF02534.13], Evalue: 2.60E-12, Aligned region: 297..489
Note	coupling protein
Protein sequence [Download]	MHPDDQRKVSAGIIIVLPFIFWITAVQKTEVLGSPKLLALWELMKLTPQKPILLLSALGG LAVGVLFVWLLNSVGQGEFGGAPFKRFLRGTRIVSGGKLKRMTREKAKQVTVAGVPMPRD AEPRHLLVNGATGTGKSVLLRELAYTGLLRGDRMVIVDPNGDMLSKFGRDKDVILNPYDK RTKGWSFFNEIRNDYDWQRYALSVVPRGKTDEAEEWASYGRLLLRETAKKLALIGTPSMR ELFHWTTIATFDDLRAFLEGTLAESLFAGSNEASKALTSARFVLSDKLPEHVTMPDGDFS IRSWLENPDGGNLFITWREDMGPALRPLISAWVDVVCTSILSLPEEPNRRLWLFIDELAS LEKLASLADALTKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASFRSLVVLGGSRTDP KTNEDMSLSLGEHEVERDRYSKNTGKHHSTGRALERVRERVVMPAEIANLPDLTAYVGFA GNRPIAKVPLEIKQFANRQPAFVEGTI

Reference
N/A

Information of plasmid

ID	108
Plasmid name	pIE321
GenBank accession number	NC_010716.1
Incompatibility group	IncW
Genome size	38150 bp
Coordinate of oriT [Strand]	8038..8368 [-]
Drug resistance	resistance to tetracycline and streptomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica serovar Dublin [98360]

Reference

[1] Revilla C et al (2008) Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids. Antimicrob Agents Chemother. 52(4):1472-80. [PMID:18268088]