oriTDB

The information of the oriT region

oriTDB accession number	100112
Name	oriT_pMdT1
Sequence Completeness	core
oriT length	64 nt
oriT sequence	AGGTTTCGGGGCGCAGCCCTGAACCAGTCACCTAGCGCTAGCGGAGTGTATACTGGCTTA GTAT
IR (inverted repeat)	IR1: [5-12] [16-23] nt (TTCGGGGC..GCCCTGAA) IR2: [32-36] [37-41] nt (CTAGC..GCTAG)
Location of nic site	[56-57]nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	ColE1 superfamily
	L:5-12;R:16-23;L:32-36;R:37-41;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGGTTTCGGGGCGCAGCCCTGAACCAGTCACCTAGCGCTAGCGGAGTGTATACTGGCTTA

61

GTAT

Reference

[1] de Toro M et al (2013) pMdT1, a small ColE1-like plasmid mobilizing a new variant of the aac(6')-Ib-cr gene in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 68(6):1277-80. [PMID:23361643]

I. Information of Relaxase

ID	107
Name	MobA_pMdT1
GenBank accession number	YP_006957893
Family	MOB_P
Length	516 aa
UniProt ID	K4I4Y4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.70E-17, Aligned region: 58..264
Note	mobilization protein MobA
Protein sequence [Download]	MPDSSGCGMPCWKRVQTMIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVREL IDASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRL ELNFLIPNMELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL PKTKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNI RLKGAIYEQSFNAGKGLRAEIESAAAEYRRDAESRIQRAREVCQSGTERKREENQRRHPR PRPEAVLSHEPAHERHGAYGQPDVADHRPGVYTADSIGRKHSMVAGAADTRQLHDHPGAE EHAGHAVREEQRRAALDLRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAER IRAATAGLLEKAGRVGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTR LNVIEAHREQERAAQHQKALELERSQRQRTYNGPSL

Reference

[1] de Toro M et al (2013) pMdT1, a small ColE1-like plasmid mobilizing a new variant of the aac(6')-Ib-cr gene in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 68(6):1277-80. [PMID:23361643]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	162	MobB_pMdT1	YP_006957895	161 aa	MobB mobilisation protein
2	163	MobC_pMdT1	YP_006957896	107 aa	MobC mobilisation protein

N/A

Information of plasmid

ID	107
Plasmid name	pMdT1
GenBank accession number	NC_019133.1
Genome size	5931 bp
Coordinate of oriT [Strand]	1978..2041 [+]
Drug resistance	resistance to tobramycin and kanamycin, and also decreased susceptibility to ciprofloxacin and norfloxacin in the transformant strain
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica serovar Typhimurium Se20 [90371]

Reference

[1] de Toro M et al (2013) pMdT1, a small ColE1-like plasmid mobilizing a new variant of the aac(6')-Ib-cr gene in Salmonella enterica serovar Typhimurium. J Antimicrob Chemother. 68(6):1277-80. [PMID:23361643]