oriTDB
The information of the oriT region
oriTDB accession number 100111
Name oriT_Rms149  insolico
Sequence Completeness incomplete
oriT length 17 nt
oriT sequence AACGGCCATCCTGTTAA
IR (inverted repeat)_
Location of nic site [13-14] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note by analogy with pTF-FC2, pTC-F14, direction inferred from R751
D: 13-14
Visualization of oriT structure
Reference
[1] Haines AS et al (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]
I. Information of Relaxase
ID 106
Name MobA_Rms149 insolico
GenBank accession number YP_245456
Family MOBP
Length 601 aa
UniProt ID Q4W1T4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.50E-30, Aligned region: 1..253
Note MobA mobilisation protein
Protein sequence [Download] MADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNPVNHYIMSWRE
GEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG
FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTG
EKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRD
ASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDK
QQEQERKQLAEQQKARRDELMRGNWNGKGEVLNAMRSVIAAEQAAEKAALKEKHKKQREQ
WRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGASGEPPTPRDIRAYQPEIVGQ
QVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVTGNDEYKA
MCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLAQLRLLDHLIAFHLTNQLHS
I
Reference
[1] Haines AS et al (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 160 MobB_Rms149 insolico YP_245458 103 aa MobB mobilisation protein
2 161 MobC_Rms149 insolico YP_245459 118 aa MobC mobilisation protein
 N/A
Information of plasmid
ID 106
Plasmid name Rms149
GenBank accession number NC_007100.1
Incompatibility group IncP(6)
Genome size 57121 bp
Coordinate of oriT  [Strand] 33033..33049 [+]
Drug resistance experimental conferring resistance to streptomycin, sulfanilamide, gentamicin, andcarbenicillin
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Pseudomonas aeruginosa Ps142 [287]
Reference
[1] Haines AS et al (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]

Microbial Bioinformatics Group in MML, SJTU