oriTDB

The information of the oriT region

oriTDB accession number	100111
Name	oriT_Rms149
Sequence Completeness	incomplete
oriT length	17 nt
oriT sequence	AACGGCCATCCTGTTAA
IR (inverted repeat)	_
Location of nic site	[13-14] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	by analogy with pTF-FC2, pTC-F14, direction inferred from R751
	D: 13-14

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AACGGCCATCCTGTTAA

Reference

[1] Haines AS et al (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]

I. Information of Relaxase

ID	106
Name	MobA_Rms149
GenBank accession number	YP_245456
Family	MOB_P
Length	601 aa
UniProt ID	Q4W1T4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.50E-30, Aligned region: 1..253
Note	MobA mobilisation protein
Protein sequence [Download]	MADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNPVNHYIMSWRE GEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTG EKSAERIAIEDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRD ASLSKLQKRLGAYQPPPQRQQVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDK QQEQERKQLAEQQKARRDELMRGNWNGKGEVLNAMRSVIAAEQAAEKAALKEKHKKQREQ WRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGASGEPPTPRDIRAYQPEIVGQ QVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVTGNDEYKA MCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLAQLRLLDHLIAFHLTNQLHS I

Reference

[1] Haines AS et al (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	160	MobB_Rms149	YP_245458	103 aa	MobB mobilisation protein
2	161	MobC_Rms149	YP_245459	118 aa	MobC mobilisation protein

N/A

Information of plasmid

ID	106
Plasmid name	Rms149
GenBank accession number	NC_007100.1
Incompatibility group	IncP(6)
Genome size	57121 bp
Coordinate of oriT [Strand]	33033..33049 [+]
Drug resistance	conferring resistance to streptomycin, sulfanilamide, gentamicin, andcarbenicillin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas aeruginosa Ps142 [287]

Reference

[1] Haines AS et al (2005) The IncP-6 plasmid Rms149 consists of a small mobilizable backbone with multiple large insertions. J Bacteriol. 187(14):4728-38. [PMID:15995187]