oriTDB
The information of the oriT region
oriTDB accession number 100109
Name oriT_pST12  insolico
Sequence Completeness intact
oriT length 242 nt
oriT sequence GCTCCGTATCTGACATGGGGTGTCGGGGCGAAGCCCTGACCAGGGTAATTGTAATAGCGT GCATGTATGCGCGGTATAACAATTGCACATCCTGTCCTGTCAGCAAGCTCGAATTACCGA CAAAGGCACGGCAGTTTTTTGGCAGTGACAGTATTGTCAGGCAGAAATACCCGTCAAAGC GTCAGACGGCAGAATACCGGCGGTAAAACAGTCGAAAAGTGGTACGCGATATAATCACAC AA
IR (inverted repeat)[22-29] [33-40] nt  (GTCGGGGC..GCCCTGAC)
Location of nic site [94-95] nt
Conserved sequence flanking the
  nic site
CATCCTG|T
Note ColE1 superfamily
L:22-29;R:33-40;D:94-95
Visualization of oriT structure
Reference
[1] Vignoli R et al (2006) New TEM-Derived Extended-Spectrum β-Lactamase and Its Genomic Context in Plasmids from Salmonella enterica Serovar Derby Isolates from Uruguay. Antimicrob Agents Chemother. 50(2):781-4. [PMID:16436745]
I. Information of Relaxase
ID 104
Name MobA_pST12 insolico
GenBank accession number YP_008574978
Family MOBP
Length 145 aa
UniProt ID T2MZH7
PDB ID _
Pfam
Note plasmid mobilization protein
Protein sequence [Download] MLTGQDVQLLYRAYMHAITITLVRASPRHPMSDTEPCFMTKRSGSNTRRRAISRPVRLTA
EEDQEIRKRAAECGKTVSGFLRAAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVG
DREYAEVLIAITEYHRALLSRLMAD
Reference
[1] Vignoli R et al (2006) New TEM-Derived Extended-Spectrum β-Lactamase and Its Genomic Context in Plasmids from Salmonella enterica Serovar Derby Isolates from Uruguay. Antimicrob Agents Chemother. 50(2):781-4. [PMID:16436745]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 156 _ insolico
 N/A
Information of plasmid
ID 104
Plasmid name pST12
GenBank accession number NC_022376.1
Genome size 8275 bp
Coordinate of oriT  [Strand] 5989..6230 [-]
Drug resistance experimental TEM-derived extended-spectrum beta-Lactamase (blaTEM gene)
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica serovar Derby str. T12 [1401650]
Reference
[1] Vignoli R et al (2006) New TEM-Derived Extended-Spectrum β-Lactamase and Its Genomic Context in Plasmids from Salmonella enterica Serovar Derby Isolates from Uruguay. Antimicrob Agents Chemother. 50(2):781-4. [PMID:16436745]