 oriTDB accession number | 100107 |
| Name | oriT_pMBUI1  |
Sequence Completeness  | core |
| oriT length | 43 nt |
| oriT sequence | GTGAAGATAGTTAACCGGCTTGCCGGTTAGCTAACTTCACCTA |
| IR (inverted repeat) | [1-19] [22-40] nt (GTGAAGATAGTTAACCGGC..GCCGGTTAGCTAACTTCAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | |
Visualization of oriT structure Reference| ID | 102 |
| Name | TraI_pMBUI1 |
| GenBank accession number | AFS51493 |
| Family | MOBP |
| Length | 802 aa |
| UniProt ID | J9Z6K5 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 4.90E-49, Aligned region: 12..252 |
| Note | TraI relaxase |
| Protein sequence [Download] | MIAKRIPMNSAKKSSFAGLVKYITSSQGINERVGQVRVTNCQTEELSWAVHEVLHTQKIN TRAEGDKTYHLLISLAPGEQPSSEVLRDIEDRVCSAIGYKDHQRVSAVHNDTDCLHIHIA INKIHPERHTLHEPYRDFKTLGDICARLELEHGLQQVNHASKKSVSEGRANDMEQHAGIE SLITWVRRECLEQVQGAKSWVELHQVMHENGLEMRERGNGLVITDQAGTMVKASTVARDL SKGKLEERLGAFENQQQQARASHKQGKPLPRKPGIGRVGRRPPPASKNRLRGLSTLQTIQ IDNGKRYEKSPLKHKSDTTELYARFRDEQNAVRHTKAKELAAARERRDRAIEQAKNVAKL KRSSIKLMATPGLTKKLLYASTHKSLQEQITSLRAEHIKAIENINTLNKARQWADWLQAK ALDGDQEALKALRARQGVKGLQGDAVAAKGQQHSAKAIPLQQDHITKEGTVIYRAGASAI RDDGSKLQLSRGANFDGIETALRMAAARYGEKITITGSDQFKELVAQTAAIRSLPIKFDD PALEQRRQSLQQAIEKERSNVGRTDRGRTAGPGAGVDGLHATRGGRGTGAGRTTGAGRGA GVRGSATGTQLSGRAGTATSGRITVRAPGGTPKPGVARVGRKPPPESQNRLRALSKLGVV QLTQGSEVLLPRDVPGVVEHQGAKPDNGLRRDVSRSGGIAAEGKTISSAQAARQYVAERE DKRAKGFDIPKHRIYNQADDGPATFAGLRQVEGQSMALLKRGDELLVLPVNEATARRMKR MKLGDPVTANATGAIKSKGRSR |
Reference| [1] Sen D et al (2013) Inferring the evolutionary history of IncP-1 plasmids despite incongruence among backbone gene trees. Mol Biol Evol. 30(1):154-66. [PMID:22936717] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 152 | TraH_pMBUI1 | AFS51494 | 139 aa | TraH DNA transfer protein |
| 2 | 153 | TraJ_pMBUI1 | AFS51495 | 140 aa | TraJ DNA transfer protein |
| 3 | 154 | TraK_pMBUI1 | AFS51496 | 142 aa | TraK oriT-binding protein |
| ID | 102 |
| Name | TraG_pMBUI1 |
| SecReT4 accesion number | _ |
| GenBank accession number | AFS51492 |
| Family | VirD4/TraG |
| Length | 638 |
| UniProt ID | Q1H9X0 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 1.10E-155, Aligned region: 123..626 TraG-D_C [PF12696.6], Evalue: 2.30E-31, Aligned region: 446..571 TrwB_AAD_bind [PF10412.8], Evalue: 4.70E-22, Aligned region: 198..582 |
| Note | TraG coupling protein |
| Protein sequence [Download] | MISKDKFNNAVGPQVRAKKPGVGKVIPALSLLSIGVGLAAATQYFAYDFKYQDVLGPHVN HIYAPWSILTWASKWYGTYPEAVMRAGSIGVLASGVGLLALAITKMIQANSSKANEYLHG SARWANKKDIQAAGLLQRGRTALEVVTSKSPPTHTGVYVGAWLDENGVQHYLRHNGPEHI LTYAPTRSGKGVGLVIPTLLSWPASTVVTDLKGELWALTSGWRQKHANNKVLRFEPATSN GGVRWNPLDEIRIGTENEVGDVQNLATLIVDPDGKGLESHWQKTSQALLVGLIMHVLYKS KIEGTLATLPEVDRILADPDRDLGELWMEMTTYGHVNGKNHPVVGSSARDMMDRPEEEAG SVLSTAKSYLSLYRDPIVARNVSASEFKVKDLMNHDNPVSLYIVTQPNDKARLRPLVRVL INMIIRLLADKMDFENGRPVAHYNHRLLAMLDEFPSLGKLEILQESLAFVAGYGIKCYLI CQDINQLKSRETGYGPDETITSNCHVQNAYPPNRVETAEHLSKLTGQTTVVKEQITTSGR RTSALLGQVSRTMQEVQRPLLTPDECLRMPGPVKSAAGDITQAGDMVVYMAGYPAIYGKQ PLYFKDPVFQARASIPAPKASDKLRFVQDHSQAEAITI |
Reference| ID | 102 |
| Plasmid name | pMBUI1 |
| GenBank accession number | JQ432563.1 |
| Incompatibility group | IncP1 |
| Genome size | 44304 bp |
| Coordinate of oriT [Strand] | 32348..32390 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Uncultured bacterium [77133] |
Reference