oriTDB

The information of the oriT region

oriTDB accession number	100106
Name	oriT_pKS208
Sequence Completeness	core
oriT length	43 nt
oriT sequence	GTGAAGAAGGCCGACCCGCTTGCGGGTGGGCCTACTTCACCTG
IR (inverted repeat)	[1-19] [22-40] nt (GTGAAGAAGGCCGACCCGC..GCGGGTGGGCCTACTTCAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note
	L:1-19;R:22-40

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGAAGAAGGCCGACCCGCTTGCGGGTGGGCCTACTTCACCTG

Reference
N/A

I. Information of Relaxase

ID	101
Name	TraI_pKS208
GenBank accession number	AFS51557
Family	MOB_P
Length	803 aa
UniProt ID	J9Z856
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.20E-51, Aligned region: 12..252
Note	TraI relaxase
Protein sequence [Download]	MISKRIPMTSAKKSSFAGLVNYITNSQGISERVGEVRITNCQSEDLRWAVGEVLSTQQQN TRAEGDKTYHLLISFAPGEKPSAEVLRDVEDRICAAMGYGEHQRVSAVHNDTDVLHIHVA INKIHPVRHTMHEPYRDFKTRSDICAKLEIEHGLERVNHAGKKPYSESRANDMERHAGVE SLLSWVRKECLEQMQGASSWKQLHQVLHEHGLELREKGNGFVIQSQDGTMVKASTVAREL SKAKLEGRLGAYEVRPSVGVQSLPAGAVPKKPGIGRVGRKPPPLSRNRLRSLGSLQSLEL DSGKRYEKRPIGNSDTTELYARYRDDQAAVRHTRTKELALERERRDRALDYAKKESALKR ASIKLMVTPGITKKLLYASAHQTLQKQLAKIRADHRKAVERINTTQKARQWADWLQAKAK DGDQEALAALRAREGARGLRGDAIAGKNKPKATTVVRAAQEHITKEGTVIYRAGASAIRD DGSKLQLSRGTNFDGIETALRMAVARYGEQITVTGSQQFKEQVAQTAALRGLPIKFDDPA LEQRRQVLQQAIEKERSNVGTDRGRAAGAGPGSDGIDTARRGRSGAGRNTTGATSGRPDG VRGYATGTGVPGRAGAGLGADGGRIAVRATGGTAKPGIARVGRKPPPESQNRLRTLSQLG VVQLTNGGESLLPSHVPGRVEQQGAATDNGLRRNVSRGGIEGLPPSIDGIKAARQYIAER EDKRAKGFDIPKHRLYNATDEGAAAFAGVRSVEGQSMALLKRGDEVLVLPINEATARRMK RMKLGEPVTTTAKGAIKSKGRSR

Reference

[1] Sen D et al (2013) Inferring the evolutionary history of IncP-1 plasmids despite incongruence among backbone gene trees. Mol Biol Evol. 30(1):154-66. [PMID:22936717]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	149	TraH_pKS208	AFS51579	142 aa	TraH DNA transfer protein
2	150	TraJ_pKS208	AFS51558	124 aa	TraJ DNA transfer protein
3	151	TraK_pKS208	AFS51559	150 aa	TraK oriT-binding protein

Information of T4CP

ID	101
Name	TraG_pKS208
SecReT4 accesion number	_
GenBank accession number	AFS51556
Family	VirD4/TraG
Length	638
UniProt ID	J9Z8Q9
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 1.40E-157, Aligned region: 123..626 TraG-D_C [PF12696.6], Evalue: 7.00E-32, Aligned region: 446..571 TrwB_AAD_bind [PF10412.8], Evalue: 3.00E-24, Aligned region: 178..579
Note	TraG coupling protein
Protein sequence [Download]	MISKDKFNNAVGPQVRAKKPGVGKLIPALSLLSIGVGLAASTQYFAYDFKYQDVLGPHYN HIYAPWSILTWANKWYGTYPDAFMRAGSIGVLATGVGLLGLVITKMIQANSSKANEYLHG SARWASKKDIQASGLLPRSRTLLEVVTGKEAPSSTGVYVGAWVDDQGVQQYLRHSGPEHV LTYAPTRSGKGVGLVVPTLLSWGASTVVTDLKGELWALTSGWRQKHARNKVLRFEPATTA GSVRWNPLDEIRIGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTSQALLVGLIMHVLYKS KNEGTPATLPEVDRILADPDRDLGELWMEMTTYSHSDGRNHPVVGSAARDMMDRPEEEAG SVLSTAKSYLALYRDPVVARNVSASEFKVKDLMNHDNPVSLFIVTQPNDKARLRPLVRVL INMIVRLLADKMDFDKGRPVAHYKHRLLMMLDEFPSLGKLEILQESLAFVAGYGIKCYLI CQDINQLKSRETGYGPDETITSNCHVQNAYPPNRVETAEHLSKLTGQTTVVKEQITTSGR RTSAMLGQVSRTMQEVQRPLLTPDECLRMPGPIKTASGDIAEAGDMVVYVAGYPAIYGKQ PLYFKDPVFQARAAIPAPKVSDKLRVVQEQAEAEAITL

Reference

[1] Sen D et al (2013) . Mol Biol Evol. 30(1):154-66. [PMID:22936717]

Information of plasmid

ID	101
Plasmid name	pKS208
GenBank accession number	JQ432564.1
Incompatibility group	IncP1
Genome size	50604 bp
Coordinate of oriT [Strand]	39342..39384 [+]
Drug resistance	bla_IMP-8
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference
N/A