 oriTDB accession number | 100106 |
| Name | oriT_pKS208  |
Sequence Completeness  | core |
| oriT length | 43 nt |
| oriT sequence | GTGAAGAAGGCCGACCCGCTTGCGGGTGGGCCTACTTCACCTG |
| IR (inverted repeat) | [1-19] [22-40] nt (GTGAAGAAGGCCGACCCGC..GCGGGTGGGCCTACTTCAC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site | _ |
| Note | |
Visualization of oriT structure Reference| ID | 101 |
| Name | TraI_pKS208 |
| GenBank accession number | AFS51557 |
| Family | MOBP |
| Length | 803 aa |
| UniProt ID | J9Z856 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.20E-51, Aligned region: 12..252 |
| Note | TraI relaxase |
| Protein sequence [Download] | MISKRIPMTSAKKSSFAGLVNYITNSQGISERVGEVRITNCQSEDLRWAVGEVLSTQQQN TRAEGDKTYHLLISFAPGEKPSAEVLRDVEDRICAAMGYGEHQRVSAVHNDTDVLHIHVA INKIHPVRHTMHEPYRDFKTRSDICAKLEIEHGLERVNHAGKKPYSESRANDMERHAGVE SLLSWVRKECLEQMQGASSWKQLHQVLHEHGLELREKGNGFVIQSQDGTMVKASTVAREL SKAKLEGRLGAYEVRPSVGVQSLPAGAVPKKPGIGRVGRKPPPLSRNRLRSLGSLQSLEL DSGKRYEKRPIGNSDTTELYARYRDDQAAVRHTRTKELALERERRDRALDYAKKESALKR ASIKLMVTPGITKKLLYASAHQTLQKQLAKIRADHRKAVERINTTQKARQWADWLQAKAK DGDQEALAALRAREGARGLRGDAIAGKNKPKATTVVRAAQEHITKEGTVIYRAGASAIRD DGSKLQLSRGTNFDGIETALRMAVARYGEQITVTGSQQFKEQVAQTAALRGLPIKFDDPA LEQRRQVLQQAIEKERSNVGTDRGRAAGAGPGSDGIDTARRGRSGAGRNTTGATSGRPDG VRGYATGTGVPGRAGAGLGADGGRIAVRATGGTAKPGIARVGRKPPPESQNRLRTLSQLG VVQLTNGGESLLPSHVPGRVEQQGAATDNGLRRNVSRGGIEGLPPSIDGIKAARQYIAER EDKRAKGFDIPKHRLYNATDEGAAAFAGVRSVEGQSMALLKRGDEVLVLPINEATARRMK RMKLGEPVTTTAKGAIKSKGRSR |
Reference| [1] Sen D et al (2013) Inferring the evolutionary history of IncP-1 plasmids despite incongruence among backbone gene trees. Mol Biol Evol. 30(1):154-66. [PMID:22936717] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 149 | TraH_pKS208 | AFS51579 | 142 aa | TraH DNA transfer protein |
| 2 | 150 | TraJ_pKS208 | AFS51558 | 124 aa | TraJ DNA transfer protein |
| 3 | 151 | TraK_pKS208 | AFS51559 | 150 aa | TraK oriT-binding protein |
| ID | 101 |
| Name | TraG_pKS208 |
| SecReT4 accesion number | _ |
| GenBank accession number | AFS51556 |
| Family | VirD4/TraG |
| Length | 638 |
| UniProt ID | J9Z8Q9 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 1.40E-157, Aligned region: 123..626 TraG-D_C [PF12696.6], Evalue: 7.00E-32, Aligned region: 446..571 TrwB_AAD_bind [PF10412.8], Evalue: 3.00E-24, Aligned region: 178..579 |
| Note | TraG coupling protein |
| Protein sequence [Download] | MISKDKFNNAVGPQVRAKKPGVGKLIPALSLLSIGVGLAASTQYFAYDFKYQDVLGPHYN HIYAPWSILTWANKWYGTYPDAFMRAGSIGVLATGVGLLGLVITKMIQANSSKANEYLHG SARWASKKDIQASGLLPRSRTLLEVVTGKEAPSSTGVYVGAWVDDQGVQQYLRHSGPEHV LTYAPTRSGKGVGLVVPTLLSWGASTVVTDLKGELWALTSGWRQKHARNKVLRFEPATTA GSVRWNPLDEIRIGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTSQALLVGLIMHVLYKS KNEGTPATLPEVDRILADPDRDLGELWMEMTTYSHSDGRNHPVVGSAARDMMDRPEEEAG SVLSTAKSYLALYRDPVVARNVSASEFKVKDLMNHDNPVSLFIVTQPNDKARLRPLVRVL INMIVRLLADKMDFDKGRPVAHYKHRLLMMLDEFPSLGKLEILQESLAFVAGYGIKCYLI CQDINQLKSRETGYGPDETITSNCHVQNAYPPNRVETAEHLSKLTGQTTVVKEQITTSGR RTSAMLGQVSRTMQEVQRPLLTPDECLRMPGPIKTASGDIAEAGDMVVYVAGYPAIYGKQ PLYFKDPVFQARAAIPAPKVSDKLRVVQEQAEAEAITL |
Reference| [1] Sen D et al (2013) . Mol Biol Evol. 30(1):154-66. [PMID:22936717] |
| ID | 101 |
| Plasmid name | pKS208 |
| GenBank accession number | JQ432564.1 |
| Incompatibility group | IncP1 |
| Genome size | 50604 bp |
| Coordinate of oriT [Strand] | 39342..39384 [+] |
| Drug resistance | blaIMP-8 |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Uncultured bacterium [77133] |
Reference