oriTDB

The information of the oriT region

oriTDB accession number	100105
Name	oriT_pCTXM360
Sequence Completeness	core
oriT length	106 nt
oriT sequence	AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGT CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
IR (inverted repeat)	[18-35] [41-58] nt (GTTTTTGGAGTACCGCCG..CGGCGGCCGTCCAAAAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L:18-35;R:41-58

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGT

61

CTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Reference
N/A

I. Information of Relaxase

ID	100
Name	MobA_pCTXM360
GenBank accession number	YP_002333288
Family	MOB_P
Length	658 aa
UniProt ID	B7T4S8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.10E-31, Aligned region: 12..333
Note	relaxase
Protein sequence [Download]	MIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRK QKVISTPVDVEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWP DYEKPNDDQVFDSVKFTLASMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSF TKDTLHQACRLLELKNGWSHSNGAYVVNDRQQIVRNPHSKKERGNWRSLDRINKMENKEG VETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHKTFADIGLRVEKAQGKKGYVIT HEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTAYTPGAYRRDAN KRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSVS DPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALS QMRGFAYSSRKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTV IFERDGKPVAADRGHIVLTESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNR IVDAAVNHNHPVAQGITFTDAAQQAYAQNEKHRLIREQNNSKNEMQFRSERDDKFNPK

Reference

[1] Zhu WH et al (2009) Complete nucleotide sequence of pCTX-M360, an intermediate plasmid between pEL60 and pCTX-M3, from a multidrug-resistant Klebsiella pneumoniae strain isolated in China. Antimicrob Agents Chemother. 53(12):5291-3. [PMID:19752275]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	147	MobB_pCTXM360	YP_002333287	105 aa	mobility protein B; conjugal transfer relaxosome component TraJ
2	148	MobC_pCTXM360	YP_002333286	121 aa	mobility protein C

N/A

Information of plasmid

ID	100
Plasmid name	pCTXM360
GenBank accession number	NC_011641.1
Incompatibility group	IncL/M
Genome size	68018 bp
Coordinate of oriT [Strand]	1896..2001 [+]
Drug resistance	bla_{CTX_M3} encoding the CTX-M-3 extended-spectrum-lactamase.
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae 0773 [573]

Reference

[1] Zhu WH et al (2009) Complete nucleotide sequence of pCTX-M360, an intermediate plasmid between pEL60 and pCTX-M3, from a multidrug-resistant Klebsiella pneumoniae strain isolated in China. Antimicrob Agents Chemother. 53(12):5291-3. [PMID:19752275]