oriTDB
The information of the oriT region
oriTDB accession number 100104
Name oriT_pAsa1  insolico
Sequence Completeness incomplete
oriT length 19 nt
oriT sequence TACGGGTGGGCTGGCTAAT
IR (inverted repeat)_
Location of nic site [14-15] nt
Conserved sequence flanking the
  nic site
CTGG|CTAA
Note ColE2-type
D:14-15
Visualization of oriT structure
Reference
[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]
I. Information of Relaxase
ID 99
Name MobA_pAsa1 insolico
GenBank accession number AAP69887
Family MOBP
Length 550 aa
UniProt ID Q79IR8
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.90E-10, Aligned region: 52..254
Note mobilization relaxase
Protein sequence [Download] MLIKFLSHGTGSGARASAYLMGSHDHTGAPRAGVAVQRGDPVLFAAVADSLPFQHRYASA
VISWATEEAPTADEITAVLEDFEAVAFAGLEPEDVHLTAVQHDEPDGGVHVHILIPRVHL
GTGKSFNPAPPGHRKAFDAVRDKHNHEKGWARPDDPLRARLLRPDFEAFKDKSDKSAIKK
QITEHLLGAAAQGVISNAAELQDYLQTSLGCEITRTGADYTSIKPEGYPKAIRLTGELYG
SSWTAERTLEREAQTAARAGIGRGGEVSESGARQAQERLRDACGRRAEYNRGRYPGRDKG
IAQTDHRLDRPIEGGLGAGPERGQHPQADLQRLAQSLAVRGMHLRGDRAGELGGIELDGQ
RGSAVEQRDQQRESHAGAASGRSELRPGGWQELHPRSDIGKNVRNAKWQDGGGDQVNDRI
RTPTDRAAGADGAAAPQRDDGLAAAALRATDVFGELAKAVGEHLGRVRDALRQLTHRIRG
LGEEHKERASQHGPEFGPDQPGYRSSTWVIEQQCGRSQELAGELDQGAALIADRAAELRK
EQRRDRGLSM
Reference
[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 144 MobB_pAsa1 insolico AAP69888 177 aa mobilization protein
2 145 MobC_pAsa1 insolico AAP69886 136 aa mobilization protein
3 146 MobD_pAsa1 insolico AAP69889 113 aa mobilization protein
 N/A
Information of plasmid
ID 99
Plasmid name pAsa1
GenBank accession number AY301063.1
Genome size 5424 bp
Coordinate of oriT  [Strand] 3260..3278 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Aeromonas salmonicida A449 [382245]
Reference
 N/A