oriTDB

The information of the oriT region

oriTDB accession number	100104
Name	oriT_pAsa1
Sequence Completeness	incomplete
oriT length	19 nt
oriT sequence	TACGGGTGGGCTGGCTAAT
IR (inverted repeat)	_
Location of nic site	[14-15] nt
Conserved sequence flanking the nic site	CTGG\|CTAA
Note	ColE2-type
	D:14-15

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TACGGGTGGGCTGGCTAAT

Reference

[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

I. Information of Relaxase

ID	99
Name	MobA_pAsa1
GenBank accession number	AAP69887
Family	MOB_P
Length	550 aa
UniProt ID	Q79IR8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.90E-10, Aligned region: 52..254
Note	mobilization relaxase
Protein sequence [Download]	MLIKFLSHGTGSGARASAYLMGSHDHTGAPRAGVAVQRGDPVLFAAVADSLPFQHRYASA VISWATEEAPTADEITAVLEDFEAVAFAGLEPEDVHLTAVQHDEPDGGVHVHILIPRVHL GTGKSFNPAPPGHRKAFDAVRDKHNHEKGWARPDDPLRARLLRPDFEAFKDKSDKSAIKK QITEHLLGAAAQGVISNAAELQDYLQTSLGCEITRTGADYTSIKPEGYPKAIRLTGELYG SSWTAERTLEREAQTAARAGIGRGGEVSESGARQAQERLRDACGRRAEYNRGRYPGRDKG IAQTDHRLDRPIEGGLGAGPERGQHPQADLQRLAQSLAVRGMHLRGDRAGELGGIELDGQ RGSAVEQRDQQRESHAGAASGRSELRPGGWQELHPRSDIGKNVRNAKWQDGGGDQVNDRI RTPTDRAAGADGAAAPQRDDGLAAAALRATDVFGELAKAVGEHLGRVRDALRQLTHRIRG LGEEHKERASQHGPEFGPDQPGYRSSTWVIEQQCGRSQELAGELDQGAALIADRAAELRK EQRRDRGLSM

Reference

[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	144	MobB_pAsa1	AAP69888	177 aa	mobilization protein
2	145	MobC_pAsa1	AAP69886	136 aa	mobilization protein
3	146	MobD_pAsa1	AAP69889	113 aa	mobilization protein

N/A

Information of plasmid

ID	99
Plasmid name	pAsa1
GenBank accession number	AY301063.1
Genome size	5424 bp
Coordinate of oriT [Strand]	3260..3278 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Aeromonas salmonicida A449 [382245]

Reference
N/A