oriTDB

The information of the oriT region

oriTDB accession number	100103
Name	oriT_pAsa3
Sequence Completeness	incomplete
oriT length	19 nt
oriT sequence	TACGGGTAGGCTGGCTTAA
IR (inverted repeat)	_
Location of nic site	[14-15] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	ColE2-type
	D:14-15

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TACGGGTAGGCTGGCTTAA

Reference

[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

I. Information of Relaxase

ID	98
Name	MobA_pAsa3
GenBank accession number	AAP69902
Family	MOB_P
Length	555 aa
UniProt ID	Q7X2D9
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.60E-08, Aligned region: 35..254
Note	mobilization relaxase
Protein sequence [Download]	MHIKFLAHGTGSGAHASAYLMGPHDHTGEPRAGVAVLRGDPVLFAAVADSLPFQQRYSSA VISWAAEEAPRPDEISAVLDDFEALAFAGLAREDVHLTAVEHAEADGGVHVHILIPRVHL GSGKSFNPAPPGQRKDFDAVRDKHNHEKGWARPDDPLRARLLRPDFEAYKAVTDHTAIKK QLTDHLIGAVAQGVVSNADELRAYLQEGLGCEITRTGKDYTSVKPEGYPKAIRLKGELYG ESWTAQSTLEREAQAAARAGIGRGGEVSASAARDAQERLAGAHQRRAEYNRDRYQGRDRG LAPADLRREQTLENSLEGGQGRSRHAETGLQRLVQSRAAGDFLRGGVTDGKLVWHELDMG ADRTRHGDVAEFAARDQQRPEHVGRVAEGGELLQGGGWKTLHHRPQDWGAVQDRGGQDGR GGALNDRAGAGTVGEAGADRTAARATNRGLEAAIAAASALFERVAKGVQRCAQELREAMQ RPARGDPDVAERGNALEQGYQRSARELEQQCQRLEQGAGELAERAGLINEQAKELKESKE LELSLEYERYRGLEL

Reference

[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	141	MobB_pAsa3	AAP69903	185 aa	mobilization protein
2	142	MobC_pAsa3	AAP69901	136 aa	mobilization protein
3	143	MobD_pAsa3	AAP69904	102 aa	mobilization protein

N/A

Information of plasmid

ID	98
Plasmid name	pAsa3
GenBank accession number	AY301065.1
Genome size	5616 bp
Coordinate of oriT [Strand]	3437..3455 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Aeromonas salmonicida A449 [382245]

Reference
N/A