oriTDB

The information of the oriT region

oriTDB accession number	100102
Name	oriT_pAsa2
Sequence Completeness	incomplete
oriT length	19 nt
oriT sequence	TCTGTGTATGCTGGCTTAG
IR (inverted repeat)	_
Location of nic site	[14-15] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	ColE1 superfamily
	D:14-15

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCTGTGTATGCTGGCTTAG

Reference

[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

I. Information of Relaxase

ID	97
Name	MobA_pAsa2
GenBank accession number	AAP69894
Family	MOB_P
Length	486 aa
UniProt ID	Q7X2E5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.20E-12, Aligned region: 46..247
Note	mobilization relaxase
Protein sequence [Download]	MIVKFHARGVGRGSGPVDYLLGPQRDREGATLDRGDPGLIEALIDSSPYAKKYTSGVLSF QEADLPREDKDKLMDSFERALLPGLDRDQYACLWVEHRDKERLELNFVIPNIELLSGKRL QPYFDRADRPRIDAWKVVVNAQLGLHDPDDPLNKRALMTPSNLPKAKQDAAQAITDGLLS LAAAGELADRGDVVATLKQHGFEVVRETRKSISIADPEGGRNIRLKGALYEQDFRAGQDL RKELEAAGERYRNEREERVRAARESYSRGVGLKRAEIERRYRRPEPEVGLAGVEGVGFSV DRGGISAGDQLGRGVVPGEGHRRELGGDLAPEQDIGAVDGEGRQIGTVNVRRGQAAVREG GLEGVGLWRQRERRISLDDPGGLLNDRARTAVIERVREFGRRVQSAALGLGERLQRFAAD VRGHLYREPGIAGERGELEQAGVALERSSREIEPKIEALSLDREPRCGGMSFGREYREYG DREYGD

Reference

[1] Boyd J et al (2003) Three small, cryptic plasmids from Aeromonas salmonicida subsp. salmonicida A449. Plasmid. 50(2):131-44. [PMID:12932739]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	138	MobB_pAsa2	AAP69895	154 aa	mobilization protein
2	139	MobC_pAsa2	AAP69893	105 aa	mobilization protein
3	140	MobD_pAsa2	AAP69896	74 aa	mobilization protein

N/A

Information of plasmid

ID	97
Plasmid name	pAsa2
GenBank accession number	AY301064.1
Genome size	5247 bp
Coordinate of oriT [Strand]	2790..2808 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Aeromonas salmonicida A449 [382245]

Reference
N/A