oriTDB
The information of the oriT region
oriTDB accession number 100100
Name oriT_pTF4.1  insolico
Sequence Completeness core
oriT length 75 nt
oriT sequence CTCCTTGCTCGGTTGGGTCAAGGGGCACCGCGCCCTTGCCTTGGGGGTCCACGGGGGAGC GGAGCATCCCCCTGG
IR (inverted repeat)IR1:[3-7] [19-23] nt  (CCTTG..CAAGG)
IR2: [49-58] [67-75] nt  (CCACGGGGGA..TCCCCCTGG)
Location of nic site [29-30]nt
Conserved sequence flanking the
  nic site 		_
Note _
L:3-7;R:19-23;L:49-58;R:67-75;D:29-30
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 95
Name ORF1_pTF4.1 insolico
GenBank accession number NP_052137
Family MOBP
Length 542 aa
UniProt ID Q56294
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.60E-11, Aligned region: 7..190
Note relaxase
Protein sequence [Download] MEAFGAGTRCKSPLYHANIDPRADERLTPEQWGRAVDALEAKLGFTGQPRALVQHVKEGR
EHVHVVWSRIDIERNRAIPDSHNYRKHEEVARALEREFGHERVQGAHVEREGKERPARTP
SHAEMQQAERSGLDPKAIKAEITALWRQTDSGKAFAGALAEAGYILARGDKRDFIVIDQA
GESHSLARRIDGVKAADMRARLSDIDQATLPDARGARAEQAQKALDDAQKAEEARTAEAR
EAAQKAAEAVERADEADLTRWRSMPLNALQWEINTLRPDRYAALAAGQAAEPTYRETLEA
TDAAHAGAVKARGEALEATETVRSLKQKAERYREYQGLLGRVRFWLQDKGILEIPEWKRL
QTEISKAQKLADKRQNDLATAEALHTEAKSALKTLDSAIAGAVDVAQAPEWRRYEVARSV
LNERIEEDRRRAAEAEAERRAEEIRRRVDAEIEREKREGIEQQRRAEISKIPTIVRGLAS
ITQTEQGTFRVRYPDLPSEELKRQQDLLRRYPDEAKSAVKAEFSERQVQAKREKDRGFDM
MD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 136 ORF4_pTF4.1 insolico NP_052136 121 aa Bacterial mobilisation protein (MobC); pfam05713
 N/A
Information of plasmid
ID 95
Plasmid name pTF4.1
GenBank accession number NC_001520.1
Genome size 4104 bp
Coordinate of oriT  [Strand] 245..319 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Acidithiobacillus ferrooxidans MAL4-1 [920]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU