oriTDB

The information of the oriT region

oriTDB accession number	100100
Name	oriT_pTF4.1
Sequence Completeness	core
oriT length	75 nt
oriT sequence	CTCCTTGCTCGGTTGGGTCAAGGGGCACCGCGCCCTTGCCTTGGGGGTCCACGGGGGAGC GGAGCATCCCCCTGG
IR (inverted repeat)	IR1:[3-7] [19-23] nt (CCTTG..CAAGG) IR2: [49-58] [67-75] nt (CCACGGGGGA..TCCCCCTGG)
Location of nic site	[29-30]nt
Conserved sequence flanking the nic site	_
Note	_
	L:3-7;R:19-23;L:49-58;R:67-75;D:29-30

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTCCTTGCTCGGTTGGGTCAAGGGGCACCGCGCCCTTGCCTTGGGGGTCCACGGGGGAGC

61

GGAGCATCCCCCTGG

Reference
N/A

I. Information of Relaxase

ID	95
Name	ORF1_pTF4.1
GenBank accession number	NP_052137
Family	MOB_P
Length	542 aa
UniProt ID	Q56294
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.60E-11, Aligned region: 7..190
Note	relaxase
Protein sequence [Download]	MEAFGAGTRCKSPLYHANIDPRADERLTPEQWGRAVDALEAKLGFTGQPRALVQHVKEGR EHVHVVWSRIDIERNRAIPDSHNYRKHEEVARALEREFGHERVQGAHVEREGKERPARTP SHAEMQQAERSGLDPKAIKAEITALWRQTDSGKAFAGALAEAGYILARGDKRDFIVIDQA GESHSLARRIDGVKAADMRARLSDIDQATLPDARGARAEQAQKALDDAQKAEEARTAEAR EAAQKAAEAVERADEADLTRWRSMPLNALQWEINTLRPDRYAALAAGQAAEPTYRETLEA TDAAHAGAVKARGEALEATETVRSLKQKAERYREYQGLLGRVRFWLQDKGILEIPEWKRL QTEISKAQKLADKRQNDLATAEALHTEAKSALKTLDSAIAGAVDVAQAPEWRRYEVARSV LNERIEEDRRRAAEAEAERRAEEIRRRVDAEIEREKREGIEQQRRAEISKIPTIVRGLAS ITQTEQGTFRVRYPDLPSEELKRQQDLLRRYPDEAKSAVKAEFSERQVQAKREKDRGFDM MD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	136	ORF4_pTF4.1	NP_052136	121 aa	Bacterial mobilisation protein (MobC); pfam05713

N/A

Information of plasmid

ID	95
Plasmid name	pTF4.1
GenBank accession number	NC_001520.1
Genome size	4104 bp
Coordinate of oriT [Strand]	245..319 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acidithiobacillus ferrooxidans MAL4-1 [920]

Reference
N/A