oriTDB

The information of the oriT region

oriTDB accession number	100099
Name	oriT_pKPC-LKEc
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTA TTACAATTACACATCCTGTCCCG
IR (inverted repeat)	IR1: [6-13] [17-24] nt (GTCGGGGC..GCCCTGAC) IR2: [31-47] [54-70] nt (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC)
Location of nic site	[78-79] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	_
	L:6-13;R:17-24;L:31-47;R:54-70;D:78-79

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTA

61

TTACAATTACACATCCTGTCCCG

Reference

[1] Chen YT et al (2014) KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 69(3):628-31. [PMID:24123430]

I. Information of Relaxase

ID	94
Name	NikB_pKPC-LKEc
GenBank accession number	AGS77392
Family	MOB_P
Length	899 aa
UniProt ID	U5KKC0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.70E-62, Aligned region: 47..315
Note	Relaxase/Mobilisation nuclease
Protein sequence [Download]	MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHY ILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYL NCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

Reference

[1] Chen YT et al (2014) KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 69(3):628-31. [PMID:24123430]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	135	NikA_pKPC-LKEc	AGS77391	110 aa	conjugal transfer relaxosome component

N/A

Information of plasmid

ID	94
Plasmid name	pKPC-LKEc
GenBank accession number	KC788405.1
Incompatibility group	IncI
Genome size	145401 bp
Coordinate of oriT [Strand]	30139..30221 [-]
Drug resistance	bla_KPC-2 (encoding beta-lactamase KPC-2)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli LK-NARMP [562]

Reference

[1] Chen YT et al (2014) KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. J Antimicrob Chemother. 69(3):628-31. [PMID:24123430]