oriTDB
The information of the oriT region
oriTDB accession number 100094
Name oriT_pNDM-40-1  insolico
Sequence Completeness incomplete
oriT length 38 nt
oriT sequence AGGGATTCATAAGGGAATTATTCCCTTATGTGGGGCTT
IR (inverted repeat)[8-17] [21-30] nt  (CATAAGGGAA..TTCCCTTATG)
Location of nic site _
Conserved sequence flanking the
  nic site
_
Note
L:8-17;R:21-30
Visualization of oriT structure
Reference
[1] Jones LS et al (2015) Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrob Agents Chemother. 59(2):923-9. [PMID:25421466]
I. Information of Relaxase
ID 89
Name TraA_pNDM-40-1 insolico
GenBank accession number AHF22521
Family MOBQ
Length 996 aa
UniProt ID W0FF73
PDB ID _
Pfam AAA_30 [PF13604.5], Evalue: 3.70E-45, Aligned region: 371..567
MobA_MobL [PF03389.14], Evalue: 1.50E-26, Aligned region: 47..237
AAA_19 [PF13245.5], Evalue: 1.70E-10, Aligned region: 376..503
Viral_helicase1 [PF01443.17], Evalue: 1.60E-05, Aligned region: 617..736
Note conjugal transfer relaxase
Protein sequence [Download] MTIAVGMHASAFLSEKQKKQKQKRHKMAIYHLSVKTGRQYGSTGTRGANHYAYINRDGKY
KRDDLENIKSGNLPSWATDAKHFWKSADKNERINGRVYTELEISLPRELNKEQREELVEK
FVEKTLGENFTYSYAIHSPLASDGQQNPHVHLMFSERKLDGIERDEVKHFKRYNPVNPEK
GGAGKDRYFSSKMFVVDTRLEWANHVNDFCENLGIDARIDHRSFKEQGIELSSQNFRADY
VSSHKYHINENIKQVRHQNGEIIIERPSEAIKALTSNQSVFSVRELERFLMAHTDGEEQY
LKAYESVITCPDMATLYGKDKVVFSSKELVGIEESISTFIYNANEESRIQKPFNLVEKLP
LNAVRVAETRTFNSEQEKAFYTLTSTDRITLLNGSAGTGKSYVLSAVSEAYKESDYQVYG
IALQAITAKAIANDCDIPSSTIASFLSKYDSGNLEINNKTVLILDEAGMVGSRDMQRLLM
LVEKHDAQIKLVGDSYQLSAVSAGHVFANIQENLDKKNIASLEDIQRQKHAKESDKMIQA
SKYLSKHEVGKAIDIYKSINMVNEYESHDLALAKTVKSWSEDSSESKLMLAHSNSDVNEL
NRMARALLIKQGKLNPESVSVKNYQGDLDISIGEKIVFKQNNSQMKVSNGELAKVIGFEK
DKFNNPKAMMVQMESDSRIVRVDLKEYDQFKHGYANTIHSSQGMTVDSAYIVASENMNAN
LTYVALTRHKQNIQINYSALTFATNEKETVKTPDGKVSYDHNYKPVEREVTAFENFKKVL
GRSETKEFSTDFTLVESKDNLMKSYLNDHRLHLENKRELFGINSKLYSLTSENKTFTKEE
IVSEVRASLKSKALLGDEIVKFNGNMNIAKGELVKLTNDLEIKTGLFKKVTFEKGTELKV
IDAYKVKDKPVMKARIKNEEYEIPFNKLNLEYSDKYFNEFKDQSKARFDSRHKTEVYTEF
REQLLKQKANAPKDNLNAPNQRIKNPNTPNRNTPKY
Reference
[1] Jones LS et al (2015) Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrob Agents Chemother. 59(2):923-9. [PMID:25421466]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 129 _ insolico
 N/A
Information of plasmid
ID 89
Plasmid name pNDM-40-1
GenBank accession number KF702385.1
Genome size 45826 bp
Coordinate of oriT  [Strand] 1..38 [+]
Drug resistance insolico harboring blaNDM-1 gene (encoding "New Delhi metal-beta-lactamase enzyme")
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Acinetobacter bereziniae CHI-40-1 [106648]
Reference
[1] Jones LS et al (2015) Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrob Agents Chemother. 59(2):923-9. [PMID:25421466]