oriTDB

The information of the oriT region

oriTDB accession number	100094
Name	oriT_pNDM-40-1
Sequence Completeness	incomplete
oriT length	38 nt
oriT sequence	AGGGATTCATAAGGGAATTATTCCCTTATGTGGGGCTT
IR (inverted repeat)	[8-17] [21-30] nt (CATAAGGGAA..TTCCCTTATG)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note
	L:8-17;R:21-30

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGGGATTCATAAGGGAATTATTCCCTTATGTGGGGCTT

Reference

[1] Jones LS et al (2015) Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrob Agents Chemother. 59(2):923-9. [PMID:25421466]

I. Information of Relaxase

ID	89
Name	TraA_pNDM-40-1
GenBank accession number	AHF22521
Family	MOB_Q
Length	996 aa
UniProt ID	W0FF73
PDB ID	_
Pfam	AAA_30 [PF13604.5], Evalue: 3.70E-45, Aligned region: 371..567 MobA_MobL [PF03389.14], Evalue: 1.50E-26, Aligned region: 47..237 AAA_19 [PF13245.5], Evalue: 1.70E-10, Aligned region: 376..503 Viral_helicase1 [PF01443.17], Evalue: 1.60E-05, Aligned region: 617..736
Note	conjugal transfer relaxase
Protein sequence [Download]	MTIAVGMHASAFLSEKQKKQKQKRHKMAIYHLSVKTGRQYGSTGTRGANHYAYINRDGKY KRDDLENIKSGNLPSWATDAKHFWKSADKNERINGRVYTELEISLPRELNKEQREELVEK FVEKTLGENFTYSYAIHSPLASDGQQNPHVHLMFSERKLDGIERDEVKHFKRYNPVNPEK GGAGKDRYFSSKMFVVDTRLEWANHVNDFCENLGIDARIDHRSFKEQGIELSSQNFRADY VSSHKYHINENIKQVRHQNGEIIIERPSEAIKALTSNQSVFSVRELERFLMAHTDGEEQY LKAYESVITCPDMATLYGKDKVVFSSKELVGIEESISTFIYNANEESRIQKPFNLVEKLP LNAVRVAETRTFNSEQEKAFYTLTSTDRITLLNGSAGTGKSYVLSAVSEAYKESDYQVYG IALQAITAKAIANDCDIPSSTIASFLSKYDSGNLEINNKTVLILDEAGMVGSRDMQRLLM LVEKHDAQIKLVGDSYQLSAVSAGHVFANIQENLDKKNIASLEDIQRQKHAKESDKMIQA SKYLSKHEVGKAIDIYKSINMVNEYESHDLALAKTVKSWSEDSSESKLMLAHSNSDVNEL NRMARALLIKQGKLNPESVSVKNYQGDLDISIGEKIVFKQNNSQMKVSNGELAKVIGFEK DKFNNPKAMMVQMESDSRIVRVDLKEYDQFKHGYANTIHSSQGMTVDSAYIVASENMNAN LTYVALTRHKQNIQINYSALTFATNEKETVKTPDGKVSYDHNYKPVEREVTAFENFKKVL GRSETKEFSTDFTLVESKDNLMKSYLNDHRLHLENKRELFGINSKLYSLTSENKTFTKEE IVSEVRASLKSKALLGDEIVKFNGNMNIAKGELVKLTNDLEIKTGLFKKVTFEKGTELKV IDAYKVKDKPVMKARIKNEEYEIPFNKLNLEYSDKYFNEFKDQSKARFDSRHKTEVYTEF REQLLKQKANAPKDNLNAPNQRIKNPNTPNRNTPKY

Reference

[1] Jones LS et al (2015) Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrob Agents Chemother. 59(2):923-9. [PMID:25421466]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	129	_

N/A

Information of plasmid

ID	89
Plasmid name	pNDM-40-1
GenBank accession number	KF702385.1
Genome size	45826 bp
Coordinate of oriT [Strand]	1..38 [+]
Drug resistance	harboring bla_NDM-1 gene (encoding "New Delhi metal-beta-lactamase enzyme")
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acinetobacter bereziniae CHI-40-1 [106648]

Reference

[1] Jones LS et al (2015) Characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in India and Pakistan. Antimicrob Agents Chemother. 59(2):923-9. [PMID:25421466]