oriTDB

The information of the oriT region

oriTDB accession number	100091
Name	oriT_pIE1120
Sequence Completeness	intact
oriT length	88 nt
oriT sequence	CCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGATTG CCGCCGCTGTGCCTCCATGATAGCCTAC
IR (inverted repeat)	[1-10] [14-23] nt (CCAGTTTCTC..GAGAAACCGG)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCG\|CCCT
Note	similar to the oriT of IncQ plasmid RSF101
	L:1-10;R:14-23;D:31-32

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGATTG

61

CCGCCGCTGTGCCTCCATGATAGCCTAC

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]

I. Information of Relaxase

ID	86
Name	MobA_pIE1120
GenBank accession number	AAC72343
Family	Other
Length	40 aa
UniProt ID	O88171
PDB ID	_
Pfam
Note	Conjugal transfer relaxase TraA; partial sequence
Protein sequence [Download]	MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVL

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	125	MobC_pIE1120	AAC72342	94 aa	mobilization protein MobC

N/A

Information of plasmid

ID	86
Plasmid name	pIE1120
GenBank accession number	AF070999.1
Incompatibility group	IncQ1
Genome size	9.1 kb
Coordinate of oriT [Strand]	203..290 [+]
Drug resistance	resistance to tetracycline and streptomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]