oriTDB
The information of the oriT region
oriTDB accession number 100091
Name oriT_pIE1120  insolico
Sequence Completeness intact
oriT length 88 nt
oriT sequence CCAGTTTCTCGAAGAGAAACCGGTAAGTGCGCCCTCCCCTACAAAGTAGGGTCGGGATTG CCGCCGCTGTGCCTCCATGATAGCCTAC
IR (inverted repeat)[1-10] [14-23] nt  (CCAGTTTCTC..GAGAAACCGG)
Location of nic site [31-32] nt
Conserved sequence flanking the
  nic site
TAAGTGCG|CCCT
Note similar to the oriT of IncQ plasmid RSF101
L:1-10;R:14-23;D:31-32
Visualization of oriT structure
Reference
[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]
I. Information of Relaxase
ID 86
Name MobA_pIE1120 insolico
GenBank accession number AAC72343
Family Other
Length 40 aa
UniProt ID O88171
PDB ID _
Pfam
Note Conjugal transfer relaxase TraA; partial sequence
Protein sequence [Download] MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVL
Reference
[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 125 MobC_pIE1120 insolico AAC72342 94 aa mobilization protein MobC
 N/A
Information of plasmid
ID 86
Plasmid name pIE1120
GenBank accession number AF070999.1
Incompatibility group IncQ1
Genome size 9.1 kb
Coordinate of oriT  [Strand] 203..290 [+]
Drug resistance experimental resistance to tetracycline and streptomycin
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Uncultured bacterium [77133]
Reference
[1] Smalla K et al (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids. Appl Environ Microbiol. 66(11):4854-62. [PMID:11055935]