oriTDB

The information of the oriT region

oriTDB accession number	100090
Name	oriT_pGO1
Sequence Completeness	core
oriT length	36 nt
oriT sequence	CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC
IR (inverted repeat)	[4-10] [14-20] nt (GCGAACG..CGTTCGC)
Location of nic site	[31-32] nt
Conserved sequence flanking the nic site	TAAGTGCGCC\|CT
Note	_
	L:4-10;R:14-20;D:31-32

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CACGCGAACGGAACGTTCGCATAAGTGCGCCCTTAC

Reference

[1] Climo MW et al (1996) Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the Staphylococcal plasmid pGO1. J Bacteriol. 178(16):4975-83. [PMID:8759863]

I. Information of Relaxase

ID	85
Name	Nes_pGO1
GenBank accession number	YP_002790904
Family	MOB_Q
Length	665 aa
UniProt ID	O87361
PDB ID	4HTE
Pfam	MobA_MobL [PF03389.14], Evalue: 7.70E-55, Aligned region: 17..218
Note	oriT relaxase Nes
Protein sequence [Download]	MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADD KFKDREYLWNKVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDL NIHKINEENPHAHLLCTLRGLDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKH LEKNGFSVRVSADSYKNQNIDLEPTKKEGWKARKFEDETGKKSSISKHNESIKKRNQQKI DKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYVTPINMYKENERLYDLKQKTS LITDDEDRLNKIEDIEDRQKKLESINEVFEKQAGIFFDKNYPDQSLNYSDDEKIFITRTI LNDRDVLPANNELEDIVKEKRIKEAQISLNTVLGNRDISLESIEKESNFFADKLSNILEK NNLSFDDVLENKHEGMEDSLKIDYYTNKLEVFRNAENILEDYYDVQIKELFTDDEDYKAF NEVTDIKEKQQLIDFKTYHGTENTIEMLETGNFIPKYSDEDRKYITEQVKLLQEKEFKPN KNQHDKFVFGAIQKKLLSEYDFDYSDNNDLKHLYQESNEVGDEISKDNIEEFYEGNDILV DKETYNNYNRKQQAYGLINAGLDSMIFNFNEIFRERMPKYINHQYKGKNHSKQRHELKNK RGMHL

Reference

[1] Climo MW et al (1996) Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the Staphylococcal plasmid pGO1. J Bacteriol. 178(16):4975-83. [PMID:8759863]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	124	_	_	_	_

Information of T4CP

ID	85
Name	TrsK_pGO1
SecReT4 accesion number	_
GenBank accession number	YP_002790929
Family	VirD4/TraG
Length	546
UniProt ID	Q07713
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 2.20E-146, Aligned region: 1..463 TraG-D_C [PF12696.6], Evalue: 6.70E-33, Aligned region: 289..414 TrwB_AAD_bind [PF10412.8], Evalue: 8.70E-18, Aligned region: 241..430
Note	similar to transfer-associated proteins of Gram-negative conjugation systems such as TraD from the F plasmid
Protein sequence [Download]	MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQS IVVTDPSGEVYEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIK SAGDSKINKDVWYKASVGLLNSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVEL DKRFNELSKDHPARESYEFGFAVSEGRTRASIILTFVADLRNYIDNEIRSYTSDSDFDLR DVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGDENGARLPVPVDFLLDEWPNI GAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVILCLGNVNNDTA KYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQ QKLEEYKEEQRQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKL KQKQAK

Reference

[1] Gomis-Rüth FX et al (2002) Identification and characterization of the origin of conjugative transfer (oriT) and a gene (nes) encoding a single-stranded endonuclease on the Staphylococcal plasmid pGO1. J Biol Chem. 277(9):7556-66. [PMID:11748238]

Information of plasmid

ID	85
Plasmid name	pGO1
GenBank accession number	NC_012547.1
Incompatibility group	Inc18
Genome size	54000 bp
Coordinate of oriT [Strand]	10191..10226 [+]
Drug resistance	aminoglycoside resistance; resistance to trimethoprim,bleomycin and quaternary ammonium compounds
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus 4220 [1280]

Reference

[1] Caryl JA et al (2009) Complete nucleotide sequence of pGO1, the prototype conjugative plasmid from the Staphylococci. Plasmid. 62(1):35-8. [PMID:19298835]

[2] Archer GL et al (1986) Plasmid-encoded trimethoprim resistance in staphylococci. Antimicrob Agents Chemother. 29(5):733-40. [PMID:3729338]