oriTDB

The information of the oriT region

oriTDB accession number	100088
Name	oriT_pRJ6
Sequence Completeness	intact
oriT length	239 nt
oriT sequence	AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATA TTCCCCAACAAGCAGGCGCGTCTGCCACACTAGTGGCTAGCAAAGCCAATGCTTGCCAAA CCACTATAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGGAGT TATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAACATATTTGTGGCTAGC
IR (inverted repeat)	IR1: [1-8] [14-21] nt (AGTGGCTA..TAGCCACT) IR2: [22-29] [34-41] nt (ATTTTTTC..GAAAAAAT) IR3: [94-98] [104-108] nt (TGGCT..AGCCA)
Location of nic site	[115-116] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	The oriT includes three MobC binding sites, a MobA binding site and a nick site. Small Staphylococcal Plasmid, ColE1 superfamily
	L:1-8;R:14-21;L:22-29;R:34-41;L:94-98;R:104-108;D:115-116

Visualization of oriT structure (The oriT was characterized experimentally)

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATA

61

TTCCCCAACAAGCAGGCGCGTCTGCCACACTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

121

CCACTATAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGGAGT

181

TATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAACATATTTGTGGCTAGC

Reference

[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

I. Information of Relaxase

ID	83
Name	MobA_pRJ6
GenBank accession number	YP_002302331
Family	MOB_P
Length	325 aa
UniProt ID	B6GUP5
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.10E-79, Aligned region: 8..242
Note	relaxase
Protein sequence [Download]	MATTKLSATKSTSRAINYAEKRAVVKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQ AFKPDEVTPEQCNQLGLELAEKFAPNHQVAVYTHNDTDHVHNHIVINSINLETGKKFNNN KQALRDLRDFNDELCRNHGLSVPEKDTARLRYTQTEKAIANPNTKSTAQYSWKDEIREAI DQSQATNIDELKTHLRQYGIEIERVTLKSITYRHLAEDKKVRGRRLGEDYNKGGIEDGFE RQIQRRQQKRRTSNRDEATERDTGITQSDWDRFAKDTNELERSRKAAESARFINEKARRD REERIQTERTTRTIINNDRDDGYEL

Reference

[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	121	MobB_pRJ6	YP_002302330	261 aa	mobilization associated protein
2	122	MobC_pRJ6	YP_002302333	127 aa	mobilization associated protein

N/A

Information of plasmid

ID	83
Plasmid name	pRJ6
GenBank accession number	NC_011522.1
Genome size	7904 bp
Coordinate of oriT [Strand]	2061..2299 [-]
Drug resistance	resistance to aureocin A70
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference

[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]