oriTDB
The information of the oriT region
oriTDB accession number 100088
Name oriT_pRJ6  experimental
Sequence Completeness intact
oriT length 239 nt
oriT sequence AGTGGCTAGCAATTAGCCACTATTTTTTCGTCAGAAAAAATCCTAAGGGGCTTGGGGATA TTCCCCAACAAGCAGGCGCGTCTGCCACACTAGTGGCTAGCAAAGCCAATGCTTGCCAAA CCACTATAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGGAGT TATCTTTCGTAGAAAGCGTGGTGGGATGTATGAGCGAACAAAACATATTTGTGGCTAGC
IR (inverted repeat)IR1: [1-8] [14-21] nt  (AGTGGCTA..TAGCCACT)
IR2: [22-29] [34-41] nt  (ATTTTTTC..GAAAAAAT)
IR3: [94-98] [104-108] nt  (TGGCT..AGCCA)
Location of nic site [115-116] nt
Conserved sequence flanking the
  nic site 		TGCTTG|CCA
Note The oriT includes three MobC binding sites, a MobA binding site and a nick site. Small Staphylococcal Plasmid, ColE1 superfamily
L:1-8;R:14-21;L:22-29;R:34-41;L:94-98;R:104-108;D:115-116
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]
I. Information of Relaxase
ID 83
Name MobA_pRJ6 insolico
GenBank accession number YP_002302331
Family MOBP
Length 325 aa
UniProt ID B6GUP5
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.10E-79, Aligned region: 8..242
Note relaxase
Protein sequence [Download] MATTKLSATKSTSRAINYAEKRAVVKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQ
AFKPDEVTPEQCNQLGLELAEKFAPNHQVAVYTHNDTDHVHNHIVINSINLETGKKFNNN
KQALRDLRDFNDELCRNHGLSVPEKDTARLRYTQTEKAIANPNTKSTAQYSWKDEIREAI
DQSQATNIDELKTHLRQYGIEIERVTLKSITYRHLAEDKKVRGRRLGEDYNKGGIEDGFE
RQIQRRQQKRRTSNRDEATERDTGITQSDWDRFAKDTNELERSRKAAESARFINEKARRD
REERIQTERTTRTIINNDRDDGYEL
Reference
[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 121 MobB_pRJ6 insolico YP_002302330 261 aa mobilization associated protein
2 122 MobC_pRJ6 insolico YP_002302333 127 aa mobilization associated protein
 N/A
Information of plasmid
ID 83
Plasmid name pRJ6
GenBank accession number NC_011522.1
Genome size 7904 bp
Coordinate of oriT  [Strand] 2061..2299 [-]
Drug resistance insolico resistance to aureocin A70
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus aureus [1280]
Reference
[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

Microbial Bioinformatics Group in MML, SJTU