oriTDB

The information of the oriT region

oriTDB accession number	100087
Name	oriT_pIP1629
Sequence Completeness	intact
oriT length	116 nt
oriT sequence	TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAATG CTTGCCAAACCACTTAACGCCTTTCTTACATTTTACTTTATAGGATAAAGTAAAAT
IR (inverted repeat)	IR1: [1-7] [11-17] nt (TTGGGGATATTCCCCAA) IR2: [30-37] [42-50] nt (GCTACCAC..GTGGCTAGC) IR3: [90-101] [105-116] nt (ATTTTACTTTAT..ATAAAGTAAAAT)
Location of nic site	[64-65] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	Small Staphylococcal Plasmid, ColE1 superfamily
	L: 1-7;R:11-17;L:30-37;R:42-50;L:90-101;R:105-116;D: 64-65

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAATG

61

CTTGCCAAACCACTTAACGCCTTTCTTACATTTTACTTTATAGGATAAAGTAAAAT

Reference

[1] Smith MC et al (2004) An accessory protein is required for relaxosome formation by small Staphylococcal plasmids. J Bacteriol. 186(11):3363-73. [PMID:15150221]

I. Information of Relaxase

ID	82
Name	MobA1_pIP1629
GenBank accession number	AAD02378
Family	MOB_P
Length	336 aa
UniProt ID	Q9Z361
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.40E-83, Aligned region: 8..242
Note	relaxase
Protein sequence [Download]	MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQ SFKPGEVTPEQCNQLGLALAEKLAPNHQVAVYTHADTDHVHNHIVMNSIDLETGKKFNNN KQALRNVRNFNDEVCMEHGLSVPDKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAI DQSQATNMNEFKDHLNQHGIEIERVTPKSITYRHLVEDKKVRGRKLGEDYNKGGIEDGFE RQIQRRRQQERDSDIECQPKRPISSRDEATKRDTGITQADWDQFAQDTNELERSRQAAES ARLADEKARRDREERERAKKASRRIINNDRDHGLEL

Reference

[1] Smith MC et al (2004) An accessory protein is required for relaxosome formation by small Staphylococcal plasmids. J Bacteriol. 186(11):3363-73. [PMID:15150221]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	119	MobB1_pIP1629	AAD02379	269 aa	mobilization protein
2	120	MobC1_pIP1629	AAD02376	127 aa	Bacterial mobilisation protein (MobC); pfam05713

N/A

Information of plasmid

ID	82
Plasmid name	pIP1629
GenBank accession number	AF045240.1
Genome size	7.5 kb
Coordinate of oriT [Strand]	395..510 [+]
Drug resistance	resistance to streptogramin A
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus epidermidis BM10385 [1282]

Reference

[1] Aubert S et al (1998) Analysis of two Staphylococcus epidermidis plasmids coding for resistance to streptogramin A. Plasmid. 40(3):238-42. [PMID:9806861]