oriTDB
The information of the oriT region
oriTDB accession number 100087
Name oriT_pIP1629  insolico
Sequence Completeness intact
oriT length 116 nt
oriT sequence TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAATG CTTGCCAAACCACTTAACGCCTTTCTTACATTTTACTTTATAGGATAAAGTAAAAT
IR (inverted repeat)IR1: [1-7] [11-17] nt  (TTGGGGATATTCCCCAA)
IR2: [30-37] [42-50] nt  (GCTACCAC..GTGGCTAGC)
IR3: [90-101] [105-116] nt  (ATTTTACTTTAT..ATAAAGTAAAAT)
Location of nic site [64-65] nt
Conserved sequence flanking the
  nic site 		TGCTTG|CCA
Note Small Staphylococcal Plasmid, ColE1 superfamily
L: 1-7;R:11-17;L:30-37;R:42-50;L:90-101;R:105-116;D: 64-65
Visualization of oriT structure
Reference
[1] Smith MC et al (2004) An accessory protein is required for relaxosome formation by small Staphylococcal plasmids. J Bacteriol. 186(11):3363-73. [PMID:15150221]
I. Information of Relaxase
ID 82
Name MobA1_pIP1629 insolico
GenBank accession number AAD02378
Family MOBP
Length 336 aa
UniProt ID Q9Z361
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.40E-83, Aligned region: 8..242
Note relaxase
Protein sequence [Download] MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQ
SFKPGEVTPEQCNQLGLALAEKLAPNHQVAVYTHADTDHVHNHIVMNSIDLETGKKFNNN
KQALRNVRNFNDEVCMEHGLSVPDKDTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAI
DQSQATNMNEFKDHLNQHGIEIERVTPKSITYRHLVEDKKVRGRKLGEDYNKGGIEDGFE
RQIQRRRQQERDSDIECQPKRPISSRDEATKRDTGITQADWDQFAQDTNELERSRQAAES
ARLADEKARRDREERERAKKASRRIINNDRDHGLEL
Reference
[1] Smith MC et al (2004) An accessory protein is required for relaxosome formation by small Staphylococcal plasmids. J Bacteriol. 186(11):3363-73. [PMID:15150221]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 119 MobB1_pIP1629 insolico AAD02379 269 aa mobilization protein
2 120 MobC1_pIP1629 insolico AAD02376 127 aa Bacterial mobilisation protein (MobC); pfam05713
 N/A
Information of plasmid
ID 82
Plasmid name pIP1629
GenBank accession number AF045240.1
Genome size 7.5 kb
Coordinate of oriT  [Strand] 395..510 [+]
Drug resistance insolico resistance to streptogramin A
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus epidermidis BM10385 [1282]
Reference
[1] Aubert S et al (1998) Analysis of two Staphylococcus epidermidis plasmids coding for resistance to streptogramin A. Plasmid. 40(3):238-42. [PMID:9806861]

Microbial Bioinformatics Group in MML, SJTU