oriTDB
The information of the oriT region
oriTDB accession number 100086
Name oriT_pIP1630  insolico
Sequence Completeness intact
oriT length 115 nt
oriT sequence TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAGTG CTTGCCAAACCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAAT
IR (inverted repeat)IR1: [1-7] [11-17] nt  (TTGGGGATATTCCCCAA)
IR2: [30-37] [42-50] nt  (GCTACCAC..GTGGCTAGC)
IR3: [89-100] [104-115] nt  (ATTTTACTTTAT..ATAAAGTAAAAT)
Location of nic site [64-65] nt
Conserved sequence flanking the
  nic site 		TGCTTG|CCA
Note Small Staphylococcal Plasmid, ColE1 superfamily
L: 1-7;R:11-17;L:30-37;R:42-50;L:89-100;R:104-115;D: 64-65
Visualization of oriT structure
Reference
[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]
I. Information of Relaxase
ID 81
Name MobA2_pIP1630 insolico
GenBank accession number AAD02405
Family MOBP
Length 337 aa
UniProt ID Q9R968
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 4.70E-83, Aligned region: 9..243
Note relaxase
Protein sequence [Download] MMATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVII
QSFKPGEVTPEQCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNN
NKQALRNVRNFNDEVCMEHGLSVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREA
IDQSQATNMDEFKDHLNQHGIEIERVTPKTITYRHLAEDKKVRGRKLGEDYNKGGIEDGF
ERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQADWDQFAQDTNELERSRQAAE
SARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL
Reference
[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 117 MobB2_pIP1630 insolico AAD02406 269 aa mobilization protein
2 118 MobC2_pIP1630 insolico AAD02403 127 aa Bacterial mobilisation protein (MobC); pfam05713
 N/A
Information of plasmid
ID 81
Plasmid name pIP1630
GenBank accession number AF045472.1
Genome size 14.4 kb
Coordinate of oriT  [Strand] 397..511 [+]
Drug resistance insolico resistance to trimethoprim
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus epidermidis BM10393 [1282]
Reference
[1] Aubert S et al (1998) Analysis of two Staphylococcus epidermidis plasmids coding for resistance to streptogramin A. Plasmid. 40(3):238-42. [PMID:9806861]

Microbial Bioinformatics Group in MML, SJTU