oriTDB

The information of the oriT region

oriTDB accession number	100086
Name	oriT_pIP1630
Sequence Completeness	intact
oriT length	115 nt
oriT sequence	TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAGTG CTTGCCAAACCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAAT
IR (inverted repeat)	IR1: [1-7] [11-17] nt (TTGGGGATATTCCCCAA) IR2: [30-37] [42-50] nt (GCTACCAC..GTGGCTAGC) IR3: [89-100] [104-115] nt (ATTTTACTTTAT..ATAAAGTAAAAT)
Location of nic site	[64-65] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	Small Staphylococcal Plasmid, ColE1 superfamily
	L: 1-7;R:11-17;L:30-37;R:42-50;L:89-100;R:104-115;D: 64-65

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTGGGGATATTCCCCAACAAGCAGGCGTCGCTACCACGTGAGTGGCTAGCAAAGCCAGTG

61

CTTGCCAAACCACTTAACGACTTGATGAATTTTACTTTATCCTATAAAGTAAAAT

Reference

[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

I. Information of Relaxase

ID	81
Name	MobA2_pIP1630
GenBank accession number	AAD02405
Family	MOB_P
Length	337 aa
UniProt ID	Q9R968
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.70E-83, Aligned region: 9..243
Note	relaxase
Protein sequence [Download]	MMATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVII QSFKPGEVTPEQCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNN NKQALRNVRNFNDEVCMEHGLSVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREA IDQSQATNMDEFKDHLNQHGIEIERVTPKTITYRHLAEDKKVRGRKLGEDYNKGGIEDGF ERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQADWDQFAQDTNELERSRQAAE SARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL

Reference

[1] Varella Coelho ML et al (2009) Mobilization functions of the bacteriocinogenic plasmid pRJ6 of Staphylococcus aureus. J Microbiol. 47(3):327-36. [PMID:19557350]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	117	MobB2_pIP1630	AAD02406	269 aa	mobilization protein
2	118	MobC2_pIP1630	AAD02403	127 aa	Bacterial mobilisation protein (MobC); pfam05713

N/A

Information of plasmid

ID	81
Plasmid name	pIP1630
GenBank accession number	AF045472.1
Genome size	14.4 kb
Coordinate of oriT [Strand]	397..511 [+]
Drug resistance	resistance to trimethoprim
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus epidermidis BM10393 [1282]

Reference

[1] Aubert S et al (1998) Analysis of two Staphylococcus epidermidis plasmids coding for resistance to streptogramin A. Plasmid. 40(3):238-42. [PMID:9806861]