oriTDB

The information of the oriT region

oriTDB accession number	100084
Name	oriT_pNM5
Sequence Completeness	core
oriT length	35 nt
oriT sequence	CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)	[1-6] [10-15] nt (CCAGAA..TTCTGG)
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	TACCGTG\|TTAT
Note	_
	L:1-6;R:10-15;D:24-25

Visualization of oriT structure

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

I. Information of Relaxase

ID	79
Name	Mob_pNM5
GenBank accession number	AFU63384
Family	MOB_V
Length	444 aa
UniProt ID	K4IDV6
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.40E-32, Aligned region: 8..187
Note	nicks plasmid at oriT to initiate conjugation
Protein sequence [Download]	MLFLTENEVSEIAHVQKYTKANMQGLSIHLDRKTENHSNEDIDIERSHLNYDLCEKEGDT LSRMNERLDEVYCMDRKDVKACCSWVVTLPENLKDAPDEHQRKFFEETYGFLADRYGGEK NVLSANVHNDETTPHMHFAFIPVVYDPKKEREKVSAKLVINRNELKVFHKDLDVHLKESI PEIYQDGILNDKTIGLDDIESIKKHSKEIEAKKADMSKELEEFSRKEKEEMVKDLATFKK PDEIIKRIDSTSKESLLGGKTNLITKDYKQLIKVAKSAIKEKQNLYNYKKVSSEKIGDLE ATVRFADRKIKNLEGKNENLEKEVAGLQEHRINEIVFKSMLQDDNRDLNISQTEIDGRLI LFNLENGHEPLDREQGERWSSTLEENKRLKTIPQSRLQRGLDKLKQFIEKFLVRGFSMDS VLQKVEQEKSRKPKNKKKSHDLEL

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	115	_

N/A

Information of plasmid

ID	79
Plasmid name	pNM5
GenBank accession number	JX863070.1
Genome size	5109 bp
Coordinate of oriT [Strand]	3401..3435 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured marine bacterium [56765]

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]