oriTDB
The information of the oriT region
oriTDB accession number 100081
Name oriT_pRAS3.1  insolico
Sequence Completeness core
oriT length 48 nt
oriT sequence CAGGATGCGGATTGTCATACACATGCTACGCATGTTCCTGCCAATCC
IR (inverted repeat)[1-18] [22-39] nt  (GGATTGGCAGGAACATGC..GCATGTGTATGACAATCC)
Location of nic site [47-48] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note
L:1-18;R:22-39;D:47-48
Visualization of oriT structure
Reference
[1] L'Abée-Lund TM et al (2002) A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas salmonicida. Plasmid. 47(3):172-81. [PMID:12151232]
I. Information of Relaxase
ID 76
Name RepB/MobA_pRAS3.1 insolico
GenBank accession number NP_387457
Family MOBP
Length 833 aa
UniProt ID Q939B3
PDB ID _
Pfam RepB_primase [PF16793.4], Evalue: 1.40E-70, Aligned region: 491..750
Relaxase [PF03432.13], Evalue: 2.90E-30, Aligned region: 6..217
Note relaxase
Protein sequence [Download] MIALSQEAVRSKDTINHYVVSWREGEQPSPEQVEEAVSIFMDELGWKDHQAIYGLHSDTD
NIHLHIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGWQREQNGRYQVLENGELG
RAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIKQAQTWEQLHRELAAKGMRY
EKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQQVAPREPEPIKPDVPG
WKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDELMRGNWKGKGEVLNAMR
SVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQKSPELAEQWRHRASEPQR
IEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVSFVDKGKSIDIHDWRNRDST
LAALQLSAQKWGSFTVMGNDEYKAMCGKLAAEHGFKITNPELQESIQQERQRIQQERVQA
MKSEQLKQFERYAEAVGAERYRVTSIKMREDGSKQTFILDKKDGITRGFTPQEIEQRTPE
MQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIRDGYQPAAVLESSPGNYQAIITV
PKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLL
KAERRECIKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAYQRHYRD
VLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRT
ARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL
Reference
[1] L'Abée-Lund TM et al (2002) A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas salmonicida. Plasmid. 47(3):172-81. [PMID:12151232]
[2] Loftie-Eaton W et al (2009) Comparative biology of two natural variants of the IncQ-2 family plasmids, pRAS3.1 and pRAS3.2. J Bacteriol. 191(20):6436-46. [PMID:19684126]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 110 MobC_pRAS3.1 insolico NP_387451 118 aa _
 N/A
Information of plasmid
ID 76
Plasmid name pRAS3.1
GenBank accession number NC_003123.1
Incompatibility group IncQ2
Genome size 11851 bp
Coordinate of oriT  [Strand] 1..48 [+]
Drug resistance insolico resistance to tetracycline
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Aeromonas salmonicida [645]
Reference
[1] L'Abée-Lund TM et al (2002) A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas salmonicida. Plasmid. 47(3):172-81. [PMID:12151232]

Microbial Bioinformatics Group in MML, SJTU