oriTDB

The information of the oriT region

oriTDB accession number	100080
Name	oriT_pBM02
Sequence Completeness	incomplete
oriT length	30 nt
oriT sequence	AAGTAAAGTATAGTGTGCTATACTTTACTT
IR (inverted repeat)	[1-12] [19-30] nt (AAGTAAAGTATA..TATACTTTACTT)
Location of nic site	[17-18] nt
Conserved sequence flanking the nic site	TAGTGTG\|CTAT
Note	homologous to that of plasmid pMV158
	L:1-12;R:19-30;D:17-18

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AAGTAAAGTATAGTGTGCTATACTTTACTT

Reference

[1] Sánchez C et al (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47. Plasmid. 49(2):118-29. [PMID:12726765]

I. Information of Relaxase

ID	75
Name	Mob_pBM02
GenBank accession number	AAK13009
Family	MOB_V
Length	304 aa
UniProt ID	Q939P5
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 4.20E-71, Aligned region: 1..193
Note	relaxase
Protein sequence [Download]	MSMMVATMKKMKNGNLGGIQKHNQREFENHSNEDIDPERSDMNYDLVNDEKINYNEKVNQ IIDEQRVGTRAIRKDAVKVDEWIISSDREFFNNLDSEQTKEFFQSVVDYFSENFGKQNVA YANVHLDETTPHMHMGVVPMKDGKLSSKTVFTREKLTEIQEQLPQYLNAKGFDIERGIEG SKSKHHDTSEYKKLKENITKEQFEDLSYKLDQSEKRNIVYQDILENDFKVKTIEPREMKA RLVLNDLERGIQPSNLDQAKEWLRNLKNAVGTKIDPSRLVKGIDKLEKIIKVVFKVVKGM TLGL

Reference

[1] Sánchez C et al (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47. Plasmid. 49(2):118-29. [PMID:12726765]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	109	_

N/A

Information of plasmid

ID	75
Plasmid name	pBM02
GenBank accession number	AY026767.1
Genome size	3854 bp
Coordinate of oriT [Strand]	248..277 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp.cremoris P8-2-47 [1359]

Reference
N/A