oriTDB
The information of the oriT region
oriTDB accession number 100080
Name oriT_pBM02  insolico
Sequence Completeness incomplete
oriT length 30 nt
oriT sequence AAGTAAAGTATAGTGTGCTATACTTTACTT
IR (inverted repeat)[1-12] [19-30] nt  (AAGTAAAGTATA..TATACTTTACTT)
Location of nic site [17-18] nt
Conserved sequence flanking the
  nic site 		TAGTGTG|CTAT
Note homologous to that of plasmid pMV158
L:1-12;R:19-30;D:17-18
Visualization of oriT structure
Reference
[1] Sánchez C et al (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47. Plasmid. 49(2):118-29. [PMID:12726765]
I. Information of Relaxase
ID 75
Name Mob_pBM02 insolico
GenBank accession number AAK13009
Family MOBV
Length 304 aa
UniProt ID Q939P5
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 4.20E-71, Aligned region: 1..193
Note relaxase
Protein sequence [Download] MSMMVATMKKMKNGNLGGIQKHNQREFENHSNEDIDPERSDMNYDLVNDEKINYNEKVNQ
IIDEQRVGTRAIRKDAVKVDEWIISSDREFFNNLDSEQTKEFFQSVVDYFSENFGKQNVA
YANVHLDETTPHMHMGVVPMKDGKLSSKTVFTREKLTEIQEQLPQYLNAKGFDIERGIEG
SKSKHHDTSEYKKLKENITKEQFEDLSYKLDQSEKRNIVYQDILENDFKVKTIEPREMKA
RLVLNDLERGIQPSNLDQAKEWLRNLKNAVGTKIDPSRLVKGIDKLEKIIKVVFKVVKGM
TLGL
Reference
[1] Sánchez C et al (2003) Sequence and analysis of pBM02, a novel RCR cryptic plasmid from Lactococcus lactis subsp cremoris P8-2-47. Plasmid. 49(2):118-29. [PMID:12726765]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 109 _ insolico
 N/A
Information of plasmid
ID 75
Plasmid name pBM02
GenBank accession number AY026767.1
Genome size 3854 bp
Coordinate of oriT  [Strand] 248..277 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp.cremoris P8-2-47 [1359]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU