oriTDB
The information of the oriT region
oriTDB accession number 100078
Name oriT_pHT beta  experimental
Sequence Completeness intact
oriT length 192 nt
oriT sequence CCAAAGAATTAATGCAAAGAGCATAAGGGAAAACTAATAGCACCTTCCTAAAGGAAGGTG GCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATCACTTCATATTTTTTGCTATCAC AAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGT TTCGTCCGTAGT
IR (inverted repeat)IR1: [38-49] [52-64] nt  (TAGCACCTTCCT..AGGAAGGTGGCTA)
IR2: [65-71] [75-81] nt  (AGTTGGC..GCCAACT)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note
L:38-49;R:52-64;L:65-71;R:75-81
Visualization of oriT structure (The oriT was characterized experimentally)
Reference
[1] Tomita H et al (2005) Genetic analysis of transfer-related regions of the vancomycin resistance Enterococcus conjugative plasmid pHTbeta: identification of oriT and a putative relaxase gene. J Bacteriol. 187(22):7727-37. [PMID:16267297]
I. Information of Relaxase
ID 73
Name ORF34_pHT beta insolico
GenBank accession number YP_398676
Family MOBP
Length 506 aa
UniProt ID Q33E54
PDB ID _
Pfam
Note putative relaxase/nickase
Protein sequence [Download] MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQEAIRQDKLDHDYSEDEL
NELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRY
ITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFLVREGYYNPETNELNENILYEATKSM
MGKMQEKEELVDPFWFATIHRNTEHIHIHVTAMERKNTREIMEYDGVLQARGKRKQSTLD
DMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLIQLYVENNSRYDKELVKYLKE
LKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDELYQTTYGKRYN
PQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAK
NDYEVAQRMIEQEKMRQAREFEIGMN
Reference
[1] Tomita H et al (2005) Genetic analysis of transfer-related regions of the vancomycin resistance Enterococcus conjugative plasmid pHTbeta: identification of oriT and a putative relaxase gene. J Bacteriol. 187(22):7727-37. [PMID:16267297]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 106 _ insolico
Information of T4CP
ID 73
Name VirD4_pHT beta insolico
SecReT4 accesion number _
GenBank accession number YP_398664
Family VirD4/TraG
Length 952
UniProt ID Q33E66
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 3.10E-17, Aligned region: 158..463
T4SS-DNA_transf [PF02534.13], Evalue: 6.10E-21, Aligned region: 519..727
TraG-D_C [PF12696.6], Evalue: 7.20E-26, Aligned region: 589..707
TrwB_AAD_bind [PF10412.8], Evalue: 2.80E-11, Aligned region: 539..717
Note Type IV secretory pathway, VirD4 component, TraG/TraD family ATPase [Intracellular trafficking, secretion, and vesicular transport]; COG3505
Protein sequence [Download] MKLVQNNEVKERKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPL
VFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYF
MCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIG
GVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPLLDNLSRAKIQSNMVVNDPKGEL
FSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWANKDYHEASKRANTLTSMLFA
SGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNELGSLFYTDPKT
GKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFN
EYLQDGDMLDIRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLK
YTDQPTAVFMVIPDYDPSNHVLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNM
PAIDNMDGIMTVCLGRNMLFDLVIQSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIE
EISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQD
TKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARF
IKDLKTRLEYSIINNIPISEEDYQDYRNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQVLDTPFIQSLINDSIA
IHNVQESKDQIMILEQQLANAYELAVLKTKNSILKNKIIKLKNAMTKLAKAI

Reference
[1] Tomita H et al (2005) Genetic analysis of transfer-related regions of the vancomycin resistance Enterococcus conjugative plasmid pHTbeta: identification of oriT and a putative relaxase gene. J Bacteriol. 187(22):7727-37. [PMID:16267297]
Information of plasmid
ID 73
Plasmid name pHT beta
GenBank accession number NC_007594.1
Genome size 52890 bp
Coordinate of oriT  [Strand] 30409..30600 [-]
Drug resistance insolico carry Tn1546-like transposons encoding vancomycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterococcus faecium [1352]
Reference
[1] Tomita H et al (2005) Genetic analysis of transfer-related regions of the vancomycin resistance Enterococcus conjugative plasmid pHTbeta: identification of oriT and a putative relaxase gene. J Bacteriol. 187(22):7727-37. [PMID:16267297]

Microbial Bioinformatics Group in MML, SJTU