oriTDB
The information of the oriT region
oriTDB accession number 100076
Name oriT2_pOLA52  insolico
Sequence Completeness incomplete
oriT length 26 nt
oriT sequence GGCGTTTGCCCTATCCTGCATCGCAG
IR (inverted repeat)_
Location of nic site [18-19] nt
Conserved sequence flanking the
  nic site
TATCCTG|C
Note oriT_pOLA52 complement(36250..36275)
D:18-7-19
Visualization of oriT structure
Reference
[1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636]
I. Information of Relaxase
ID 71
Name TaxC_pOLA52 (IncX1) insolico
GenBank accession number YP_001693207
Family MOBP
Length 388 aa
UniProt ID B0ZDZ0
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.30E-10, Aligned region: 64..306
Note relaxase
Protein sequence [Download] MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGA
VTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENK
KITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRD
NDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKGVY
EVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLKKL
GQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQQQ
RMAQFTQQKASTLQSEQKLKTGIKFFNI
Reference
[1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 103 TaxA_pOLA52 insolico YP_001693208 181 aa auxilary protein; similar to TaxA from R6K
Information of T4CP
ID 71
Name TaxB_pOLA52 insolico
SecReT4 accesion number _
GenBank accession number YP_001693193
Family VirD4/TraG
Length 611
UniProt ID B0ZDX6
PDB ID _
Pfam T4SS-DNA_transf [PF02534.13], Evalue: 3.50E-143, Aligned region: 101..578
TraG-D_C [PF12696.6], Evalue: 2.90E-24, Aligned region: 415..535
TrwB_AAD_bind [PF10412.8], Evalue: 1.20E-16, Aligned region: 307..551
Note putative T4SS coupling protein; similar to TaxB from R6K
Protein sequence [Download] MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIR
TDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKEN
DTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKI
TSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTGAE
AHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSKRE
ELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTDGN
DFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL
DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAV
SEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFI
ILKGESPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKML
SVGELESEVLL

Reference
 N/A
Information of plasmid
ID 71
Plasmid name pOLA52
GenBank accession number NC_010378.1
Incompatibility group IncX1
Genome size 51602 bp
Coordinate of oriT  [Strand] 30771..30796 [+]; 36250..36275 [-]
Drug resistance insolico resistance-nodulation-division (RND)-type efflux-pump (oqxAB); bla encoding beta-lactamase TEM precursor
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli [562]
Reference
[1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636]