oriTDB

The information of the oriT region

oriTDB accession number	100075
Name	oriT1_pOLA52
Sequence Completeness	incomplete
oriT length	26 nt
oriT sequence	GGCGTTTGCCCTATCCTGCATCGCAG
IR (inverted repeat)	_
Location of nic site	[18-19] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	oriT_pOLA52 (30771..30796)
	D:18-7-19

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGCGTTTGCCCTATCCTGCATCGCAG

Reference

[1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636]

I. Information of Relaxase

ID	71
Name	TaxC_pOLA52 (IncX1)
GenBank accession number	YP_001693207
Family	MOB_P
Length	388 aa
UniProt ID	B0ZDZ0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.30E-10, Aligned region: 64..306
Note	relaxase
Protein sequence [Download]	MGVYVDKEHRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINRKGASKEVVVKISGGA VTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKEHMIDRANDPQHVMNDKGKENK KITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYHCDKKEHPHVHVVFRIRD NDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLNQSVKDAHNTAPKRQKGVY EVVDIGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFGDLCSRESVKAGDLVRLKKL GQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRTPSASKELVLNSPDMLLKQQQ RMAQFTQQKASTLQSEQKLKTGIKFFNI

Reference

[1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	103	TaxA_pOLA52	YP_001693208	181 aa	auxilary protein; similar to TaxA from R6K

Information of T4CP

ID	71
Name	TaxB_pOLA52
SecReT4 accesion number	_
GenBank accession number	YP_001693193
Family	VirD4/TraG
Length	611
UniProt ID	B0ZDX6
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 3.50E-143, Aligned region: 101..578 TraG-D_C [PF12696.6], Evalue: 2.90E-24, Aligned region: 415..535 TrwB_AAD_bind [PF10412.8], Evalue: 1.20E-16, Aligned region: 307..551
Note	putative T4SS coupling protein; similar to TaxB from R6K
Protein sequence [Download]	MSLKLPDKGQWAFIGLVMCLVTYYTGSVAVYFLNGKTPLYIWKNFDSMLLWRIITESNIR TDIRLTAIPSLLSGMVSSLIVPVFIIWQLNKTDVALYGDAKFASDNDLRKSKLLKWEKEN DTDILVGAYKGKYLWYTAPDFVSLGAGTRAGKGAAIGIPNLLVRKHSLIALDPKQELWKI TSKVREILLGNKVYLLDPFNSKTHQFNPLFYIDLKAESGAKDLLKLIEILFPSYGMTGAE AHFNNLAGQYWTGLAKLLHFFINYEPSWLNEFGLKPVFSIGSVVDLYSNIDRELILSKRE ELEGTNGLDENALYHLRDALTKIREYHETEDEQRSSIDGSFRKKMSLFYLPTVRKCTDGN DFDLRQLRREDITVYVGVNAEDISLAYDFLNLFFNFVVEVTLRENPDFDPTLKHDCLMFL DEFPSIGYMPIIKKGSGYIAGFKLKLLTIYQNISQLNEIYGIEGAKTLMSAHPCRIIYAV SEEDDAAKISEKLGYITTTSKSTSKSRGRSTSQGESESEARRALVLPQELGTLDFKEEFI ILKGESPVKAEKALYYLDPYFMDRLMKVSPKLASLTMELNKTKKIFGVKGLKYPSKEKML SVGELESEVLL

Reference
N/A

Information of plasmid

ID	71
Plasmid name	pOLA52
GenBank accession number	NC_010378.1
Incompatibility group	IncX1
Genome size	51602 bp
Coordinate of oriT [Strand]	30771..30796 [+]; 36250..36275 [-]
Drug resistance	resistance-nodulation-division (RND)-type efflux-pump (oqxAB); bla encoding beta-lactamase TEM precursor
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli [562]

Reference

[1] Norman A et al (2008) Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid. 60(1):59-74. [PMID:18440636]