oriTDB

The information of the oriT region

oriTDB accession number	100073
Name	oriT_pMA67
Sequence Completeness	core
oriT length	35 nt
oriT sequence	CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)	[1-6] [10-15] nt (CCAGAA..TTCTGG)
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	TACCGTG\|TTAT
Note	_
	L:1-6;R:10-15;D:24-25

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT

Reference

[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]

I. Information of Relaxase

ID	69
Name	Mob_pMA67
GenBank accession number	YP_001966010
Family	MOB_V
Length	435 aa
UniProt ID	A0SGK6
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 7.20E-36, Aligned region: 1..182
Note	nicks plasmid at oriT to initiate conjugation
Protein sequence [Download]	MAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEV YCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDE TTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILND KTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVV MLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYRNIDFG REITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKNKEEGE NWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKELSQQRH DRRKRKADLVAWSCN

Reference

[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	100	_

N/A

Information of plasmid

ID	69
Plasmid name	pMA67
GenBank accession number	NC_010875.1
Genome size	5030 bp
Coordinate of oriT [Strand]	3425..3459 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Paenibacillus larvae 67E [1464]

Reference

[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]