oriTDB
The information of the oriT region
oriTDB accession number 100073
Name oriT_pMA67  insolico
Sequence Completeness core
oriT length 35 nt
oriT sequence CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)[1-6] [10-15] nt  (CCAGAA..TTCTGG)
Location of nic site [24-25] nt
Conserved sequence flanking the
  nic site 		TACCGTG|TTAT
Note _
L:1-6;R:10-15;D:24-25
Visualization of oriT structure
Reference
[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]
I. Information of Relaxase
ID 69
Name Mob_pMA67 insolico
GenBank accession number YP_001966010
Family MOBV
Length 435 aa
UniProt ID A0SGK6
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 7.20E-36, Aligned region: 1..182
Note nicks plasmid at oriT to initiate conjugation
Protein sequence [Download] MAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDERLSEV
YCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSANVHNDE
TTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQEGILND
KTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFNKKEVV
MLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYRNIDFG
REITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKNKEEGE
NWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKELSQQRH
DRRKRKADLVAWSCN
Reference
[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 100 _ insolico
 N/A
Information of plasmid
ID 69
Plasmid name pMA67
GenBank accession number NC_010875.1
Genome size 5030 bp
Coordinate of oriT  [Strand] 3425..3459 [+]
Drug resistance insolico resistance to tetracycline
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Paenibacillus larvae 67E [1464]
Reference
[1] You Y et al (2012) Detection of a common and persistent tet(L)-carrying plasmid in chicken-waste-impacted farm soil. Appl Environ Microbiol. 78(9):3203-13. [PMID:22389375]

Microbial Bioinformatics Group in MML, SJTU