oriTDB

The information of the oriT region

oriTDB accession number	100071
Name	oriT_pNL194
Sequence Completeness	incomplete
oriT length	10 nt
oriT sequence	GGTGTATAGC
IR (inverted repeat)	_
Location of nic site	[5-6] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	putative IncN oriT nick site
	D:5-6

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTGTATAGC

Reference

[1] Miriagou V et al (2010) Sequence of pNL194, a 79.3-kilobase IncN plasmid carrying the bla_VIM-1 metallo-beta-lactamase gene in Klebsiella pneumoniae. Antimicrob Agents Chemother. 54(10):4497-502. [PMID:20660690]

I. Information of Relaxase

ID	67
Name	TraI_pNL194
GenBank accession number	YP_003813077
Family	MOB_F
Length	1078 aa
UniProt ID	E2EAU3
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 6.70E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 4.30E-42, Aligned region: 477..663 Viral_helicase1 [PF01443.17], Evalue: 5.00E-07, Aligned region: 774..899 UvrD_C_2 [PF13538.5], Evalue: 1.10E-08, Aligned region: 851..897
Note	conjugal nickase and helicase
Protein sequence [Download]	MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLV GEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREA EKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALK NDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQIE KGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQGH GKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIANKQVLGHATI ADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKK EQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALED DGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQ TKAVEAGKPFEQLIKAGMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELK EEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRV DSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVESSSGEQIAFSP RTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTD DKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFN QKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQ WSAQEYADYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI

Reference

[1] Miriagou V et al (2010) Sequence of pNL194, a 79.3-kilobase IncN plasmid carrying the bla_VIM-1 metallo-beta-lactamase gene in Klebsiella pneumoniae. Antimicrob Agents Chemother. 54(10):4497-502. [PMID:20660690]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	98	TraK_pNL194	YP_003813079	138 aa	_

Information of T4CP

ID	67
Name	TraG_pNL194
SecReT4 accesion number	302141619
GenBank accession number	YP_003813031
Family	TrwB/TraD
Length	331
UniProt ID	E2EAP7
PDB ID	_
Pfam	T2SSE [PF00437.19], Evalue: 2.20E-43, Aligned region: 8..312
Note	Type IV Secretion System Coupling Protein
Protein sequence [Download]	MTDAAFYQLGPLREYLEDPTVFEIRINCFQEVICDTFSGRRVVQNAAITADFIRNLAKSL VSSNKLTMQAINDVILPGGIRGVICLPPAVIDGTTAVAFRKDLAADKNLEQLTSEGIFSD CRKITGSKQSLTDDDFFLKELHSSEKWPAFLQTAVEKKRTIVICGETGSGKTVLTRALLK SLHKDERVIILEDVHEVTVDHVVEAVYMMYGDAGKIGPVSATDALRACMRLTPGRIIMTE LRDDAAWDYLKALNTGHPGGVMSTHANSARDAFNRIGLLIKATPIGRMLDMSDIMRMLYS TIDVVVHMEKRKIKEIYFDPEYKMQCVNGSL

Reference

[1] Miriagou V et al (2010) Sequence of pNL194, a 79.3-kilobase IncN plasmid carrying the bla_VIM-1 metallo-beta-lactamase gene in Klebsiella pneumoniae. Antimicrob Agents Chemother. 54(10):4497-502. [PMID:20660690]

Information of plasmid

ID	67
Plasmid name	pNL194
GenBank accession number	NC_014368.1
Incompatibility group	IncN
Genome size	79307 bp
Coordinate of oriT [Strand]	59504..59513 [+]
Drug resistance	carrying a mosaic structure including bla_VIM-1, aacA7, aadA1, aadA2, dfrA1, dfrA12, aphA1, strA, strB, and sul1.
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae NL194 [573]

Reference

[1] Miriagou V et al (2010) Sequence of pNL194, a 79.3-kilobase IncN plasmid carrying the bla_VIM-1 metallo-beta-lactamase gene in Klebsiella pneumoniae. Antimicrob Agents Chemother. 54(10):4497-502. [PMID:20660690]