oriTDB
The information of the oriT region
oriTDB accession number 100067
Name oriT_pPsv48B  insolico
Sequence Completeness intact
oriT length 107 nt
oriT sequence CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTTGC TAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCG TGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGGTAGCGCTTCGCTTGTCGTTTGT CATCAGTCGATAAATCAATTGCGTCATTGATCGTCGGAGGC
IR (inverted repeat)IR1: [26-41] [51-66] nt  (CGATGTATACGGGGGG..CCCCCCGAATACATCG)
IR2: [73-80] [84-91] nt  (CAAAGGGG..CCCCTTTG)
Location of nic site _
Conserved sequence flanking the
  nic site 		_
Note
L:26-41;R: 51-66;L:73-80;R:84;91
Visualization of oriT structure
Reference
[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]
I. Information of Relaxase
ID 63
Name MobB_pPsv48B insolico
GenBank accession number YP_006961623
Family MOBP
Length 1257 aa
UniProt ID H1ZX26
PDB ID _
Pfam
Note putative relaxase, mobilization protein
Protein sequence [Download] MLIRPGGYNTGAQEYLEEGNKSGREFTRDELDHRLVISGDLDLTRSIYESIPDRGQDRYL
TFTLSFREDAVAESTLKAVTAEFKQFLMYAYKAEEFNFYAEAHLPKIKYVTDKKTGKPVE
RKPHIHVIVPRINLLSGNEANPVGFYKNHEKYFEAFQEYLNQKYNLASPREHVRVDIADA
ASVLSRYKGDDFYGKNREFKQTLVKQVIEKNVTTREAFYELAATYGETRIRNQGKDNEYV
AVKLPGDAKFTNLKETIFHDDFIVRRDLKKEPLDKAIIAQRLTEWPQRAMEIKYVEKATP
AFRKRYVAASPEERQQLLAEREQKFYQVHGEHNDNVHTGQRQRDHQRSPSETAGRRTAAP
ADGLQDLSVSDVADHRQAGSARSRDGALLLPSDAHVHLGQSQPGGDSGLRPSVPGGGRGR
GSRSTTERGRGGSGPAAVSQEATGTATPTGATGRRRAGAGKPRNSRVRAGRVVPPYAKNP
HRVATIADIEERGRRLFDPLKRPSDNALVFYRAPTVASVPKAATATATPGRSTAGRRPRT
SGKPRPPRQWRPGAVVPPYAKNPHRVATVADIEQRARMLFDPLKRHADKALVFKRASIKA
LTVNKQASTVASYFTRQAQHNQIAPAHRRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLV
SVLTFERIKAREQFHKPQPNTEVNFMGSAAIRNLLDDEKEDPGFSISGARGPGPEGVRDR
VKRVMDRFAKQVDPAAASQRARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVDTGKTIS
MRRTGITEAGVSVALQLARERFGSTLTITGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSL
AARRAELDIERGGQSIGPATDLPRHVDDSTRDVRDQADRMGVTVPIEALYGEGKTAEQVS
QALATQLDTVPEPERIAFVETVAITLGIPERCQPKGDQAFAQWQAQRAQPAADSAATATS
EAQTRVATPSDAAPEPVSPTASTNPAPASPEAAKPALANDPDLQSPSELVRLEAQWRRDF
PMSEADVRASDTVMGLRGEDHAVWIIATNDKTPEAAAMLASYMENDSYREAFKASIVAAY
KQVENSPKLVDDLDHLTAMAAQIVNEVEERLFPTPQAATGQTAPSRSKVIEGTLIEHGEA
PYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDAGLKQGDQVRLQDLGTQPVVVQV
IEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSMD
Reference
[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 94 MobC_pPsv48B insolico YP_006961622 116 aa putative relaxosome component
 N/A
Information of plasmid
ID 63
Plasmid name pPsv48B
GenBank accession number NC_019266.1
Genome size 45220 bp
Coordinate of oriT  [Strand] 37888..37994 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor insolico pPsv48B contains a putative T3SS effector identified as hopAO1 (sin. avrPphD2) which functions as a suppressor of plant resistance triggered by PAMPS.
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Pseudomonas savastanoi NCPPB 3335 [29438]
Reference
[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]

Microbial Bioinformatics Group in MML, SJTU