oriTDB

The information of the oriT region

oriTDB accession number	100067
Name	oriT_pPsv48B
Sequence Completeness	intact
oriT length	107 nt
oriT sequence	CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTTGC TAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCG TGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGGTAGCGCTTCGCTTGTCGTTTGT CATCAGTCGATAAATCAATTGCGTCATTGATCGTCGGAGGC
IR (inverted repeat)	IR1: [26-41] [51-66] nt (CGATGTATACGGGGGG..CCCCCCGAATACATCG) IR2: [73-80] [84-91] nt (CAAAGGGG..CCCCTTTG)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note
	L:26-41;R: 51-66;L:73-80;R:84;91

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTGTGTCATCGAGAGACATTCAGAGACAATCTTGGGTCTGACCCAAAATCTCGAATTTGC

61

TAAACAGGCACGTTACCGATGTATACGGGGGGATGAAGCGCCCCCCCGAATACATCGCCG

121

TGCCAAAGGGGAAACCCCTTTGAAACCCCGCAGAGCGGTAGCGCTTCGCTTGTCGTTTGT

181

CATCAGTCGATAAATCAATTGCGTCATTGATCGTCGGAGGC

Reference

[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]

I. Information of Relaxase

ID	63
Name	MobB_pPsv48B
GenBank accession number	YP_006961623
Family	MOB_P
Length	1257 aa
UniProt ID	H1ZX26
PDB ID	_
Pfam
Note	putative relaxase, mobilization protein
Protein sequence [Download]	MLIRPGGYNTGAQEYLEEGNKSGREFTRDELDHRLVISGDLDLTRSIYESIPDRGQDRYL TFTLSFREDAVAESTLKAVTAEFKQFLMYAYKAEEFNFYAEAHLPKIKYVTDKKTGKPVE RKPHIHVIVPRINLLSGNEANPVGFYKNHEKYFEAFQEYLNQKYNLASPREHVRVDIADA ASVLSRYKGDDFYGKNREFKQTLVKQVIEKNVTTREAFYELAATYGETRIRNQGKDNEYV AVKLPGDAKFTNLKETIFHDDFIVRRDLKKEPLDKAIIAQRLTEWPQRAMEIKYVEKATP AFRKRYVAASPEERQQLLAEREQKFYQVHGEHNDNVHTGQRQRDHQRSPSETAGRRTAAP ADGLQDLSVSDVADHRQAGSARSRDGALLLPSDAHVHLGQSQPGGDSGLRPSVPGGGRGR GSRSTTERGRGGSGPAAVSQEATGTATPTGATGRRRAGAGKPRNSRVRAGRVVPPYAKNP HRVATIADIEERGRRLFDPLKRPSDNALVFYRAPTVASVPKAATATATPGRSTAGRRPRT SGKPRPPRQWRPGAVVPPYAKNPHRVATVADIEQRARMLFDPLKRHADKALVFKRASIKA LTVNKQASTVASYFTRQAQHNQIAPAHRRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLV SVLTFERIKAREQFHKPQPNTEVNFMGSAAIRNLLDDEKEDPGFSISGARGPGPEGVRDR VKRVMDRFAKQVDPAAASQRARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVDTGKTIS MRRTGITEAGVSVALQLARERFGSTLTITGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSL AARRAELDIERGGQSIGPATDLPRHVDDSTRDVRDQADRMGVTVPIEALYGEGKTAEQVS QALATQLDTVPEPERIAFVETVAITLGIPERCQPKGDQAFAQWQAQRAQPAADSAATATS EAQTRVATPSDAAPEPVSPTASTNPAPASPEAAKPALANDPDLQSPSELVRLEAQWRRDF PMSEADVRASDTVMGLRGEDHAVWIIATNDKTPEAAAMLASYMENDSYREAFKASIVAAY KQVENSPKLVDDLDHLTAMAAQIVNEVEERLFPTPQAATGQTAPSRSKVIEGTLIEHGEA PYQHNDDNQMSYFVTLKPEGGKPRTVWGVGLEEAMSDAGLKQGDQVRLQDLGTQPVVVQV IEEDGTVTDKTVNRREWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSMD

Reference

[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	94	MobC_pPsv48B	YP_006961622	116 aa	putative relaxosome component

N/A

Information of plasmid

ID	63
Plasmid name	pPsv48B
GenBank accession number	NC_019266.1
Genome size	45220 bp
Coordinate of oriT [Strand]	37888..37994 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	pPsv48B contains a putative T3SS effector identified as hopAO1 (sin. avrPphD2) which functions as a suppressor of plant resistance triggered by PAMPS.
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas savastanoi NCPPB 3335 [29438]

Reference

[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]