oriTDB

The information of the oriT region

oriTDB accession number	100065
Name	oriT_pPsv48C
Sequence Completeness	intact
oriT length	146 nt
oriT sequence	TAGGTGCAGTGTGCACTTGTCTTTTGGAACCGTACTCGAAATCCGAATTCGCTAAACAGG CACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCA AAGGGGATACCCCTTTGGAACCCCGCAGAGCGGGAAAGCGTTGTTGTTGTCGTTGTTGGT TGTCGTCATTCCAGGTGCCCCCAGGAGGTGATCTTGTGGC
IR (inverted repeat)	IR1: [38-43] [52-57] nt (AAAAAG..CTTTTT) IR2: [72-83] [87-98] nt (GTGCCAAAGGGG..CCCCTTTGGAAC)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note
	L:38-43;R:52-57;L:72-83;R:87-98

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TAGGTGCAGTGTGCACTTGTCTTTTGGAACCGTACTCGAAATCCGAATTCGCTAAACAGG

61

CACGTTACCGATTGTCTACAAAAAAGAGCTTCGCCTTTTTCGTATCCAATCGACGTGCCA

121

AAGGGGATACCCCTTTGGAACCCCGCAGAGCGGGAAAGCGTTGTTGTTGTCGTTGTTGGT

181

TGTCGTCATTCCAGGTGCCCCCAGGAGGTGATCTTGTGGC

Reference

[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]

I. Information of Relaxase

ID	61
Name	MobB_pPsv48C
GenBank accession number	YP_006962036
Family	MOB_P
Length	1254 aa
UniProt ID	H1ZX53
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 8.90E-07, Aligned region: 9..267
Note	putative relaxase, mobilization protein
Protein sequence [Download]	MLIRVSGYNTGAQEYLEKGNKSGREFTRDELDHRLIIEGQLSLTRAIYESIPDYGQDRYL TFALSFKEDIVSPELLKAVTNDVKNFLMHAYKPEEFNFYAEAHLPKMKTITDRKTDEVID RKPHIHIIIPRINLLSGNEANPVDVYKNHEKYFEAIQEHINQKYGLSSPRENVRADITDA ASVLSRYKGDDFYGKNRQFKQELVKQVIERGVTSRADFYALVAEHGETRIRNQGKDTEYI SVKLPGDAKGTNLKDTIFQDDFIVRRELKKPPLEASVIQERLLAWPQRAREIKYVNKATP KFRKQYSEASPEDRVRLLADREAKFYQVHGEHNDNVHNGQRQRDHQRSSAETAGRRTSAP ADGLQDLSVSHVADHRQAGSARSRDGAVLLPSDAHVHVGQSQPGGDSGLRPSVPGGGRGR RNGSTTERGRGGSGPAAVSQEATGTATPAGATGRRRTGAGKPRNARVRAGRIIPPYAKNP HRVATIADIEERGRRLFDPLKKPSDNALVFYRAPSNTPATATTTSGRSSAGRKPRTSGKP RPPRQWRPGSVVPPYAKNPHRVATVADIEQRARMLFDPLKRPADKALVFKRASIKALTVN RQASTVAAYFTREAQHNQIAPAHRRAIRRIDQQYFALRRAVFSDQRLTRQDKAQLVSVLT FERLKAREQFHNPQPKTEVNFMGSAAIRNLLDDEKEDPGFSISGARGPGPEGVRDRVKRV MDRFAKQVDPVAASERARDLSAKDLYTRKAKFSQNVHYLDKQTDKTLFVDTGTTISMRRT GITEAGVSVALQLARERFGSTLTINGTAEFKKLVIEAVAKNGLDVHFTDKAMNQSLADRR AELDIERDGQSIGPATDLPRHVDDATRDVRNQADRLSVTVPIEALYGEGKTAEQVSLALA TQLDTVPEPERIAFVELVAITLGIPERGEPKGDQAFAQWQAQRAQPAADSTATATSEAQA NVPTASDTAPEAVTPTAATSPASSASPDAAKPARLNDPDLQSPSELVRLEAQWRRDFPMS EADVRASDTVMGLRGEDHAVWIIATNDKTPEAAAMLTAYMENDSYREAFKASIVAAYKQV ENSPKLVDDLDHLTAMAAQIVNEVEDRLYPEPQSASAQATPPRSKVIEGTLIEHGEAPYQ HKDTNQLSYFVTLKPEGGKPRTVWGVGLEEAMTDANLKQGNQVRLEDLGTWPVVVQVIAE DGTTSDKKVNRRAWSAQPVAPEREVAETTPKGQAAAAGTPELSSPDEEDGMSMD

Reference

[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	92	MobC_pPsv48C	YP_006962035	241 aa	putative relaxosome component

N/A

Information of plasmid

ID	61
Plasmid name	pPsv48C
GenBank accession number	NC_019292.1
Genome size	42103 bp
Coordinate of oriT [Strand]	21090..21235 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	pPsv48C contains a CDS with significant homology to isopentenyl-diphosphate delta-isomerase,type 1 (InterPro family IPR011876), which could participate in cytokinins biosynthesis.
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Pseudomonas savastanoi NCPPB 3335 [29438]

Reference

[1] Bardaji L et al (2011) Sequence and role in virulence of the three plasmid complement of the model tumor-inducing bacterium Pseudomonas savastanoi pv. savastanoi NCPPB 3335. PLoS One. 6(10):e25705. [PMID:22022435]