oriTDB

The information of the oriT region

oriTDB accession number	100063
Name	oriT_pBHS24
Sequence Completeness	core
oriT length	35 nt
oriT sequence	CCAGAAGAATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)	[1-6] [10-15] nt (CCAGAA..TTCTGG)
Location of nic site	[24-25] nt
Conserved sequence flanking the nic site	TACCGTG\|TTAT
Note	_
	L:1-6;R:10-15;D:24-25

Visualization of oriT structure

Reference

[1] Phelan RW et al (2011) Tetracycline resistance-encoding plasmid from Bacillus sp. strain #24, isolated from the marine sponge Haliclona simulans. Appl Environ Microbiol. 77(1):327-9. [PMID:21057017]

I. Information of Relaxase

ID	59
Name	Mob_pBHS24
GenBank accession number	YP_006960887
Family	MOB_V
Length	439 aa
UniProt ID	E2G6N9
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note	nicks plasmid at oriT to initiate conjugation
Protein sequence [Download]	MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL SQQRQQQKKTKSRSSGMEL

Reference

[1] Phelan RW et al (2011) Tetracycline resistance-encoding plasmid from Bacillus sp. strain #24, isolated from the marine sponge Haliclona simulans. Appl Environ Microbiol. 77(1):327-9. [PMID:21057017]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	90	_

N/A

Information of plasmid

ID	59
Plasmid name	pBHS24
GenBank accession number	NC_019230.1
Genome size	5031 bp
Coordinate of oriT [Strand]	3425..3459 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacillus sp. 24 [886284]

Reference

[1] Phelan RW et al (2011) Tetracycline resistance-encoding plasmid from Bacillus sp. strain #24, isolated from the marine sponge Haliclona simulans. Appl Environ Microbiol. 77(1):327-9. [PMID:21057017]