 oriTDB accession number | 100061 |
| Name | oriT_N3  |
Sequence Completeness  | incomplete |
| oriT length | 48 nt |
| oriT sequence | TAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGC |
| IR (inverted repeat) | [1-7] [19-25] nt (TAAATCA..TGATTTA) |
| Location of nic site | [43-44] nt |
| Conserved sequence flanking the nic site | GGTGT|ATAGC |
| Note | _ |
Visualization of oriT structure Reference| [1] Humphrey B et al (2012) Fitness of Escherichia coli strains carrying expressed and partially silent IncN and IncP1 plasmids. BMC Microbiol. 0.536805556. [PMID:22475035] |
| ID | 57 |
| Name | TraI_N3 |
| GenBank accession number | YP_004558187 |
| Family | MOBF |
| Length | 1080 aa |
| UniProt ID | F6IB82 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 6.70E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 4.30E-42, Aligned region: 477..663 Viral_helicase1 [PF01443.17], Evalue: 5.10E-07, Aligned region: 774..899 UvrD_C_2 [PF13538.5], Evalue: 1.10E-08, Aligned region: 851..897 |
| Note | conjugal transfer nickase and helicase |
| Protein sequence [Download] | MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLV GEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREA EKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALK NDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQIE KGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQGH GKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIANKQVLGHATI ADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKK EQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALED DGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQ TKAVEAGKPFEQLIKAGMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELK EEAPRLSQLADRYLSLSSEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRV DSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVESSSGEQIAFSP RTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTD DKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFN QKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQ WSAQEYADYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEHEEGGHEI |
Reference| [1] Humphrey B et al (2012) Fitness of Escherichia coli strains carrying expressed and partially silent IncN and IncP1 plasmids. BMC Microbiol. 0.536805556. [PMID:22475035] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 88 | TraK_N3 | YP_004558185 | 138 aa | _ |
| ID | 57 |
| Name | TraJ_N3 |
| SecReT4 accesion number | 336053209 |
| GenBank accession number | YP_004558186 |
| Family | TrwB/TraD |
| Length | 509 |
| UniProt ID | F6IB81 |
| PDB ID | _ |
| Pfam | TrwB_AAD_bind [PF10412.8], Evalue: 7.00E-84, Aligned region: 112..491 TraG-D_C [PF12696.6], Evalue: 3.50E-19, Aligned region: 350..472 T4SS-DNA_transf [PF02534.13], Evalue: 1.30E-09, Aligned region: 297..500 DUF87 [PF01935.16], Evalue: 2.70E-05, Aligned region: 109..272 |
| Note | Type IV Secretion System Coupling Protein |
| Protein sequence [Download] | MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIA GWFIGVGGLIAFIIYDKSRVFKGERFKKIYRGTELVSARTLADKTRERGVNQLTVANIPI PTYAENLHFSIAGTTGTGKTTIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNA YDKRTEGWVFFNEIRRSYDYERLVNSIVQESPDMATEEWFGYGRLIFSEVSKKLHSLYST VTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGSARFVLSKNLAPHLKMPEGNF SLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESRINVFIDELES LQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALP GDMPVAKFKAKHVKYHRKNPVPGIELRDI |
Reference| [1] Humphrey B et al (2012) Fitness of Escherichia coli strains carrying expressed and partially silent IncN and IncP1 plasmids. BMC Microbiol. 0.536805556. [PMID:22475035] |
| ID | 57 |
| Plasmid name | N3 |
| GenBank accession number | NC_015599.1 |
| Incompatibility group | IncN |
| Genome size | 54205 bp |
| Coordinate of oriT [Strand] | 10313..10360 [-] |
| Drug resistance | tet(A) genes for tetracycline resistance, a putative bacA-like bacitracin resistance gene |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli 345-2RifC [562] |
Reference| [1] Humphrey B et al (2012) Fitness of Escherichia coli strains carrying expressed and partially silent IncN and IncP1 plasmids. BMC Microbiol. 0.536805556. [PMID:22475035] |