oriTDB

The information of the oriT region

oriTDB accession number	100053
Name	oriT_pKLH80
Sequence Completeness	intact
oriT length	117 nt
oriT sequence	TAAGCCAGCAGTCACAAGAGGGGGCTATTTTATGCTACGCTAAAAACATCCTATCCCTCT TGTGTGCTGGTTGGGGAGACCCCAAACCCCCTTCAATCTTTTAAGGTGCGTCTTATT
IR (inverted repeat)	IR: [28-32] [41-45] nt (TTTTA..TAAAA) [71-76] [80-85] nt (TTGGGG..CCCCAA) [102-105] [112-115] nt (TAAG..CTTA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	_
	L: 28-32;R:41-45;L:71-76;R:80-85;L:102-105;R:112-115

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TAAGCCAGCAGTCACAAGAGGGGGCTATTTTATGCTACGCTAAAAACATCCTATCCCTCT

61

TGTGTGCTGGTTGGGGAGACCCCAAACCCCCTTCAATCTTTTAAGGTGCGTCTTATT

Reference

[1] Petrova M et al (2014) Genetic structure and biological properties of the first ancient multiresistance plasmid pKLH80 isolated from a permafrost bacterium. Microbiology. 160(Pt 10):2253-63. [PMID:25063046]

I. Information of Relaxase

ID	51
Name	MobA_pKLH80
GenBank accession number	CCW43458
Family	MOB_P
Length	388 aa
UniProt ID	N1NUY4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.80E-08, Aligned region: 9..289
Note	relaxase/mobilization nuclease domain protein,MobA
Protein sequence [Download]	MIVKFSKHGKGKASGVLNYLLKEKGSDGVLVPRKHTKVLYGDPVLTEHLIDTAVHKSKYK SGYLSFSERADEISEDDKKRIMQEFEKVIFCGLEPDQYDILWVEHADKDIDDNNPVGRLE LNFVIPCQELRTGKSFQPYYEPADQRRVNAWKNIINAEVKTINAERLSDPNDPKRQRLVN PYNSHAPRPSPFNINSYSKKQSDEDDEIIQHPKSRRDLEEALKRRLLLESQRGVVINRQS LLTTLRRWGLNVNRSNSETSVSVKHDDLKDKNGRVMSVRLTGGMFAKNYRGIEFKPYIQE VNSNNYYSEPNKTHRSQLDKMNLEEGIKIKAQYHQDRYKEATLPEPLAIQKAKVTNIVEE AVDPKGAEQLKYNTPGKLDQKIVANKIE

Reference

[1] Petrova M et al (2014) Genetic structure and biological properties of the first ancient multiresistance plasmid pKLH80 isolated from a permafrost bacterium. Microbiology. 160(Pt 10):2253-63. [PMID:25063046]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	82	MobC_pKLH80	CCW43459	121 aa	_

N/A

Information of plasmid

ID	51
Plasmid name	pKLH80
GenBank accession number	HF953351.1
Incompatibility group	IncP1
Genome size	14835 bp
Coordinate of oriT [Strand]	1..117 [-]
Drug resistance	genes encoding resistance to streptomycin and tetracycline; carbenicillin-hydrolysing beta-lactamase gene: bla_RTG-6
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Psychrobacter maritimus MR29-12 [256325]

Reference

[1] Petrova M et al (2014) Genetic structure and biological properties of the first ancient multiresistance plasmid pKLH80 isolated from a permafrost bacterium. Microbiology. 160(Pt 10):2253-63. [PMID:25063046]