 oriTDB accession number | 100052 |
| Name | oriT_pIFM3804  |
Sequence Completeness  | intact |
| oriT length | 110 nt |
| oriT sequence | CCTGAAAAACGGGACAGGATGTGCAATTGTAATACCGTCACACGCGACGCTATTACAATT GCCATCTGGTCAGGGCTTCGCCCCGACACCCCGTAAGGAGCCTGAAGTGA |
| IR (inverted repeat) | 8-bp: [24-31] [35-42] nt (GTCGGGGC..GCCCTGAC) 17-bp: [49-65] [72-88] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | [96-97] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | similar to oriT of plasmid R64 |
Visualization of oriT structure Reference| [1] Freire Martín I et al (2014) Sequence analysis of a CTX-M-1 IncI1 plasmid found in Salmonella 4,5,12:i:-, Escherichia coli and Klebsiella pneumoniae on a UK pig farm. J Antimicrob Chemother. 69(8):2098-101. [PMID:24729584] |
| ID | 50 |
| Name | NikB_pIFM3804 |
| GenBank accession number | YP_008998777 |
| Family | MOBP |
| Length | 899 aa |
| UniProt ID | E9LLN1 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 9.20E-64, Aligned region: 47..315 |
| Note | Relaxase/Mobilisation nuclease |
| Protein sequence [Download] | MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG |
Reference| [1] Freire Martín I et al (2014) Sequence analysis of a CTX-M-1 IncI1 plasmid found in Salmonella 4,5,12:i:-, Escherichia coli and Klebsiella pneumoniae on a UK pig farm. J Antimicrob Chemother. 69(8):2098-101. [PMID:24729584] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 81 | NikA_pIFM3804 | YP_008998776 | 110 aa | _ |
| ID | 50 |
| Name | TrbC_pIFM3804 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_008998778 |
| Family | VirD4/TraG |
| Length | 763 |
| UniProt ID | E9LLN2 |
| PDB ID | _ |
| Pfam | |
| Note | nucleotide-binding protein |
| Protein sequence [Download] | MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISV DAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQI SVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY |
Reference| ID | 50 |
| Plasmid name | pIFM3804 |
| GenBank accession number | NC_023329.1 |
| Incompatibility group | IncI1 |
| Genome size | 104399 bp |
| Coordinate of oriT [Strand] | 47883..47992 [-] |
| Drug resistance | blaCTX-M-1 gene |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli B3804 [562] |
Reference| [1] Freire Martín I et al (2014) Sequence analysis of a CTX-M-1 IncI1 plasmid found in Salmonella 4,5,12:i:-, Escherichia coli and Klebsiella pneumoniae on a UK pig farm. J Antimicrob Chemother. 69(8):2098-101. [PMID:24729584] |