oriTDB

The information of the oriT region

oriTDB accession number	100051
Name	oriT_pPL373
Sequence Completeness	core
oriT length	34 nt
oriT sequence	CCAGAAGATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)	[1-6] [9-14] nt (CCAGAA..TTCTGG)
Location of nic site	[23-24] nt
Conserved sequence flanking the nic site	TACCGTG\|TTAT
Note	_
	L:1-6;R:9-14;D:23-24

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCAGAAGATTCTGGTATACCGTGTTATACCAAAT

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

I. Information of Relaxase

ID	49
Name	Mob_pPL373
GenBank accession number	AGX24949
Family	MOB_V
Length	439 aa
UniProt ID	U5LK84
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note	nicks plasmid at oriT to initiate conjugation
Protein sequence [Download]	MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL SQQRQQQKKTKSRSSGMEL

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	80	_

N/A

Information of plasmid

ID	49
Plasmid name	pPL373
GenBank accession number	KF433938.1
Genome size	5030 bp
Coordinate of oriT [Strand]	3425..3458 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Paenibacillus larvae PL373 [1464]

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]