oriTDB
The information of the oriT region
oriTDB accession number 100050
Name oriT_pPL395  insolico
Sequence Completeness core
oriT length 34 nt
oriT sequence CCAGAAGATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)[1-6] [9-14] nt  (CCAGAA..TTCTGG)
Location of nic site [23-24] nt
Conserved sequence flanking the
  nic site
TACCGTG|TTAT
Note _
L:1-6;R:9-14;D:23-24
Visualization of oriT structure
Reference
[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]
I. Information of Relaxase
ID 48
Name Mob_pPL395 insolico
GenBank accession number AGX24954
Family MOBV
Length 439 aa
UniProt ID U5LK84
PDB ID _
Pfam Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note nicks plasmid at oriT to initiate conjugation
Protein sequence [Download] MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE
RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN
VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE
GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN
KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR
NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN
KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL
SQQRQQQKKTKSRSSGMEL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 79 _ insolico
 N/A
Information of plasmid
ID 48
Plasmid name pPL395
GenBank accession number KF440690.1
Genome size 5030 bp
Coordinate of oriT  [Strand] 3425..3458 [+]
Drug resistance insolico resistance to tetracycline
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Paenibacillus larvae PL395 [1464]
Reference
[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]