oriTDB

The information of the oriT region

oriTDB accession number	100049
Name	oriT_pPL374
Sequence Completeness	core
oriT length	34 nt
oriT sequence	CCAGAAGATTCTGGTATACCGTGTTATACCAAAT
IR (inverted repeat)	[1-6] [9-14] nt (CCAGAA..TTCTGG)
Location of nic site	[23-24] nt
Conserved sequence flanking the nic site	TACCGTG\|TTAT
Note	_
	L:1-6;R:9-14;D:23-24

Visualization of oriT structure

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

I. Information of Relaxase

ID	47
Name	Mob_pPL374
GenBank accession number	AGX24957
Family	MOB_V
Length	439 aa
UniProt ID	U5LK84
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 5.30E-37, Aligned region: 1..187
Note	nicks plasmid at oriT to initiate conjugation
Protein sequence [Download]	MEVYEMAHVQKFTKGNMQGLSIHLDRKTENHSNKNIDTERTHLNYDLCEKDGDTLSRMDE RLSEVYCMNRKDVKVCCEWIVTLPESLHNESVEYQRKFFEETYEFLTDRYGGQENVVSAN VHNDETTPHMHFDFVPVVWDEKKEREKVSAKEVLTRSELKSFHTDLDRHLKERIPEIYQE GILNDKTIGIENIHSLKKYSAEIEQQKEEMANEFKSFKYPQKVLKSIEREVEKKTVGVFN KKEVVMLPAERYEKMKELSNSSIRIQQRFDKYKSKAVEEIKNLKVNVKNFEEDNEHLRYR NIDFGREITLLQKERDRQTENAIVYKSILEEKEPDLQISTLEFQGRLVLHNLENDRMPKN KEEGENWLEILEENKEEKTIPQNRLEKAIGKIKLFLEKFIKRAKEADFSMDWLVEKNKEL SQQRQQQKKTKSRSSGMEL

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	78	_

N/A

Information of plasmid

ID	47
Plasmid name	pPL374
GenBank accession number	KF536616.1
Genome size	5026 bp
Coordinate of oriT [Strand]	3424..3457 [+]
Drug resistance	resistance to tetracycline
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Paenibacillus larvae PL374 [1464]

Reference

[1] Alippi AM et al (2014) Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys. Int Microbiol. 17(1):49-61. [PMID:25296446]