 oriTDB accession number | 100047 |
| Name | oriT_pGS18  |
Sequence Completeness  | core |
| oriT length | 34 nt |
| oriT sequence | GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG |
| IR (inverted repeat) | [4-18] [22-34] nt (CCCATTTATACCCCT..AGGGGTAAATGGG) |
| Location of nic site | [2-3]nt |
| Conserved sequence flanking the nic site | _ |
| Note | _ |
Visualization of oriT structure Reference| [1] Stuknyte M et al (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896] |
| ID | 45 |
| Name | ORF55_pGS18 |
| GenBank accession number | YP_001716052 |
| Family | MOBQ |
| Length | 643 aa |
| UniProt ID | B1GSL9 |
| PDB ID | _ |
| Pfam | MobA_MobL [PF03389.14], Evalue: 4.50E-71, Aligned region: 16..223 |
| Note | relaxase |
| Protein sequence [Download] | MAYFLFRTQIINKADRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPE WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD RINEKFKEKGINDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEKQGKPYEPVTY YGKLNQEIQRINQELKALKQQEKVVSISEYQEEKSLNETIESIRKNASLNDVEKASLLLV AKRAKSYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVYQQEPRQVIQY GFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH AGADFRIEEKYHAVQYFKDTGSVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVL NRTIQKQSKERMEALKVRDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYG RDVSITNTAALLRLHELVERGESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYR QHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER |
Reference| [1] Stuknyte M et al (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 75 | _ | _ | _ |
| ID | 45 |
| Name | TrsK_pGS18 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_001716053 |
| Family | VirD4/TraG |
| Length | 711 |
| UniProt ID | B1GUT7 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 3.00E-90, Aligned region: 97..596 TrwB_AAD_bind [PF10412.8], Evalue: 4.70E-42, Aligned region: 140..543 TraG-D_C [PF12696.6], Evalue: 1.20E-33, Aligned region: 404..527 |
| Note | Type IV secretory system Conjugative DNA transfer |
| Protein sequence [Download] | MNLKKDAWKPIFASVVLTVIAAYVLMGLFYMIGNGGDLKEILLRPKELITFVYTEENLKT VFSLAPFVILAALLYTFRSSIIVPKLEYASDFGLHGTSRWGTPDEVIDGKMFSKNNSYTT DPVDAFQMEKGIIVGKVPKKRELLIIPRNTTIDNRNVLVIGSSGSGKGQSFVFPNMINHT EETIIVTDPKGEIYEATHQIKRDQGYKVYQVDFINFVNCDRYNPLDYVEDDEDARAIANT IASNSVDEGKRDFWSESAVAFLSAFILYVKEKYKDKANMRHVVEMVARAGKDEEYLDEII KNMSPDHPAYHMFTLANMSSGNTRAGIMATLAQQISIFAMQKIAKMTAKSDFKFHDLQKE KSVLYIKIRMDENPFVQLTATFFEQLIAVLYDIADKNHSKLPIPTIFLLDEFANIGTINK YPRVLATCRGLGMAMMTIIQDIGQLEDKKRYGPEMARSIINNHDTQLFLRTKDTKTAKYF SELAGETTVKHKQKSASYGRKEGSKSISEQYVKRSLITPGELMNMDKNTCYLFVSGAYPM KLEKAWQFDIYGDLLSNYEKYRSRLGYTAPLWNGEWVWDEEDSAAQDIQEVKQELEESDV VLLNEMIDEINKIDEDEEDEEITEEELRVLLGNVEDENEDEENESEEVGEAEELQSILDE IEEELDAFDEMEDEITEEDLMEIIEEFSEFSDDSEGEQEESNQKIADELPI |
Reference| [1] Stuknyte M et al (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896] |
| ID | 45 |
| Plasmid name | pGS18 |
| GenBank accession number | NC_010420.1 |
| Genome size | 62830 bp |
| Coordinate of oriT [Strand] | 50079..50112 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Geobacillus stearothermophilus 18 [1422] |
Reference