oriTDB

The information of the oriT region

oriTDB accession number	100047
Name	oriT_pGS18
Sequence Completeness	core
oriT length	34 nt
oriT sequence	GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG
IR (inverted repeat)	[4-18] [22-34] nt (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site	[2-3]nt
Conserved sequence flanking the nic site	_
Note	_
	L:4-18;R:22-34;D:2-3

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG

Reference

[1] Stuknyte M et al (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896]

I. Information of Relaxase

ID	45
Name	ORF55_pGS18
GenBank accession number	YP_001716052
Family	MOB_Q
Length	643 aa
UniProt ID	B1GSL9
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 4.50E-71, Aligned region: 16..223
Note	relaxase
Protein sequence [Download]	MAYFLFRTQIINKADRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPE WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD RINEKFKEKGINDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEKQGKPYEPVTY YGKLNQEIQRINQELKALKQQEKVVSISEYQEEKSLNETIESIRKNASLNDVEKASLLLV AKRAKSYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVYQQEPRQVIQY GFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH AGADFRIEEKYHAVQYFKDTGSVLPESEIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVL NRTIQKQSKERMEALKVRDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYG RDVSITNTAALLRLHELVERGESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYR QHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER

Reference

[1] Stuknyte M et al (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	75	_	_	_

Information of T4CP

ID	45
Name	TrsK_pGS18
SecReT4 accesion number	_
GenBank accession number	YP_001716053
Family	VirD4/TraG
Length	711
UniProt ID	B1GUT7
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 3.00E-90, Aligned region: 97..596 TrwB_AAD_bind [PF10412.8], Evalue: 4.70E-42, Aligned region: 140..543 TraG-D_C [PF12696.6], Evalue: 1.20E-33, Aligned region: 404..527
Note	Type IV secretory system Conjugative DNA transfer
Protein sequence [Download]	MNLKKDAWKPIFASVVLTVIAAYVLMGLFYMIGNGGDLKEILLRPKELITFVYTEENLKT VFSLAPFVILAALLYTFRSSIIVPKLEYASDFGLHGTSRWGTPDEVIDGKMFSKNNSYTT DPVDAFQMEKGIIVGKVPKKRELLIIPRNTTIDNRNVLVIGSSGSGKGQSFVFPNMINHT EETIIVTDPKGEIYEATHQIKRDQGYKVYQVDFINFVNCDRYNPLDYVEDDEDARAIANT IASNSVDEGKRDFWSESAVAFLSAFILYVKEKYKDKANMRHVVEMVARAGKDEEYLDEII KNMSPDHPAYHMFTLANMSSGNTRAGIMATLAQQISIFAMQKIAKMTAKSDFKFHDLQKE KSVLYIKIRMDENPFVQLTATFFEQLIAVLYDIADKNHSKLPIPTIFLLDEFANIGTINK YPRVLATCRGLGMAMMTIIQDIGQLEDKKRYGPEMARSIINNHDTQLFLRTKDTKTAKYF SELAGETTVKHKQKSASYGRKEGSKSISEQYVKRSLITPGELMNMDKNTCYLFVSGAYPM KLEKAWQFDIYGDLLSNYEKYRSRLGYTAPLWNGEWVWDEEDSAAQDIQEVKQELEESDV VLLNEMIDEINKIDEDEEDEEITEEELRVLLGNVEDENEDEENESEEVGEAEELQSILDE IEEELDAFDEMEDEITEEDLMEIIEEFSEFSDDSEGEQEESNQKIADELPI

Reference

[1] Stuknyte M et al (2008) Complete nucleotide sequence of pGS18, a 62.8-kb plasmid from Geobacillus stearothermophilus strain 18. Extremophiles. 12(3):415-29. [PMID:18305896]

Information of plasmid

ID	45
Plasmid name	pGS18
GenBank accession number	NC_010420.1
Genome size	62830 bp
Coordinate of oriT [Strand]	50079..50112 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Geobacillus stearothermophilus 18 [1422]

Reference
N/A