oriTDB

The information of the oriT region

oriTDB accession number	100044
Name	oriT_pTcM1
Sequence Completeness	intact
oriT length	286 nt
oriT sequence	TCCTGTCGTTCCTCCTGTGCCGGTTTTCTTAAGCTTTGCATAGATTCTACTTCATTGACT TTCGTAAAGAAAGCACTACAATACGTATAAGCTATATTTTGAACAGGATGGGACTTGTCA TACACATGCTACGCATGTTCCCGCCAAGCCACGCCCAACCATTCGGCGCTACGCTTCTCA CGGTCCGGCTCATCCTGCAAAGGGCAAGCCCCTTGACCCCGGCCAGCGCGGCGCCATGGC TTCTTGCTGACCCAGACACACCCCGCCGCCGCGAAAGGAGACACGA
IR (inverted repeat)	[59-63] [69-73] nt (CTTTC..GAAAG) [124-129] [133-138] nt (ACATGC..GCATGT)
Location of nic site	[197-198] nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	_
	L:59-63;R:69-73;L:124-129;R:133-138;D:197-198

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCTGTCGTTCCTCCTGTGCCGGTTTTCTTAAGCTTTGCATAGATTCTACTTCATTGACT

61

TTCGTAAAGAAAGCACTACAATACGTATAAGCTATATTTTGAACAGGATGGGACTTGTCA

121

TACACATGCTACGCATGTTCCCGCCAAGCCACGCCCAACCATTCGGCGCTACGCTTCTCA

181

CGGTCCGGCTCATCCTGCAAAGGGCAAGCCCCTTGACCCCGGCCAGCGCGGCGCCATGGC

241

TTCTTGCTGACCCAGACACACCCCGCCGCCGCGAAAGGAGACACGA

Reference

[1] van Zyl LJ et al (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]

I. Information of Relaxase

ID	42
Name	MobL_pTcM1
GenBank accession number	YP_001837312
Family	MOB_Q
Length	928 aa
UniProt ID	B1A4K1
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.40E-15, Aligned region: 46..237
Note	relaxase/mobilization nuclease domain-containing protein
Protein sequence [Download]	MAIYHLHAKTWSKGAGQGAGGHARYVLRQGPYARRVIERVDGSAVRRVEEDRAAEVVASY SGHLPAWADGDPLAYWDAADAYERANGTVYRELEFALPGELSERRNLKLAQAFAEALARV DRGATPYTLVLHRSTRDPELLHCHLMLSDRVNDGYERSPELWFRRAANRGKDPARGGAPK TQARIAQDWLGTVVRPLWAELANAALEQARCGARIDHRSLEAQRRERELLAEQAQGRGDE LAAARYRKAAGILDRPAQPKRGRVLEHGGPDKAPGQALVWARYQEDLARRRAARERLAEA DGVVRELREEIARERRREERRAAAKRELDASHGWRPDRVATAQMVLAAVLDLGQAHREAV QERWDARRERRRVAIEAQRRVEQERQERRGQLLGLAASLAADAECRLRLREAYEAAGFRL EQRPDGKWVFVYLHGDLLEERQAVREARRAWDERRAALEAQRAAAREAEERRRREGERDR ATEQEQGPGVRHPDKPRWQVERELILTEVYGSAVAEHMGRWYRIERRADALVLSNREASV TDFGDRVVAHEGNDKEIEALVLLAQAKGWERVTLTGPREFQERAAQAFVKAGIALEDQAL EARAKEAIERQRREDIRERALLRQGYRRDAWEIRERWHWRQQERARRLEAERELARIEEE AEQARDAWRTVWRKTGHLVDADAQEPIPWGIWREQTLAERYGPDIAARAEAEYWYTRLRP DLGGLDISRNGQEVVDAGAALQVCSAGSMSLAVDLVRAKGWKRVAIGGPEDFRERAARAL IEAGMVLADEALEQRAKAALDAERVQQERERALRRAMEGIKAHRIRWRRKDGTMGEQRAL DYLEDRLFRGFTIQETAEGFRWWSADGKPGAVVGDAQLMALFRAAKAADQDLVRVVERIR MEVGMPNAAADRARGRTVTPKRGLGDDW

Reference

[1] van Zyl LJ et al (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	70	_	_	_	_

N/A

Information of plasmid

ID	42
Plasmid name	pTcM1
GenBank accession number	NC_010600.1
Genome size	65158 bp
Coordinate of oriT [Strand]
Drug resistance	_
Heavy-metal resistance	arsenic resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Acidithiobacillus caldus MNG [33059]

Reference

[1] van Zyl LJ et al (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]