oriTDB
The information of the oriT region
oriTDB accession number 100044
Name oriT_pTcM1  insolico
Sequence Completeness intact
oriT length 286 nt
oriT sequence TCCTGTCGTTCCTCCTGTGCCGGTTTTCTTAAGCTTTGCATAGATTCTACTTCATTGACT TTCGTAAAGAAAGCACTACAATACGTATAAGCTATATTTTGAACAGGATGGGACTTGTCA TACACATGCTACGCATGTTCCCGCCAAGCCACGCCCAACCATTCGGCGCTACGCTTCTCA CGGTCCGGCTCATCCTGCAAAGGGCAAGCCCCTTGACCCCGGCCAGCGCGGCGCCATGGC TTCTTGCTGACCCAGACACACCCCGCCGCCGCGAAAGGAGACACGA
IR (inverted repeat)[59-63] [69-73] nt  (CTTTC..GAAAG)
[124-129] [133-138] nt  (ACATGC..GCATGT)
Location of nic site [197-198] nt
Conserved sequence flanking the
  nic site 		CATCCTG|C
Note _
L:59-63;R:69-73;L:124-129;R:133-138;D:197-198
Visualization of oriT structure
Reference
[1] van Zyl LJ et al (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]
I. Information of Relaxase
ID 42
Name MobL_pTcM1 insolico
GenBank accession number YP_001837312
Family MOBQ
Length 928 aa
UniProt ID B1A4K1
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 2.40E-15, Aligned region: 46..237
Note relaxase/mobilization nuclease domain-containing protein
Protein sequence [Download] MAIYHLHAKTWSKGAGQGAGGHARYVLRQGPYARRVIERVDGSAVRRVEEDRAAEVVASY
SGHLPAWADGDPLAYWDAADAYERANGTVYRELEFALPGELSERRNLKLAQAFAEALARV
DRGATPYTLVLHRSTRDPELLHCHLMLSDRVNDGYERSPELWFRRAANRGKDPARGGAPK
TQARIAQDWLGTVVRPLWAELANAALEQARCGARIDHRSLEAQRRERELLAEQAQGRGDE
LAAARYRKAAGILDRPAQPKRGRVLEHGGPDKAPGQALVWARYQEDLARRRAARERLAEA
DGVVRELREEIARERRREERRAAAKRELDASHGWRPDRVATAQMVLAAVLDLGQAHREAV
QERWDARRERRRVAIEAQRRVEQERQERRGQLLGLAASLAADAECRLRLREAYEAAGFRL
EQRPDGKWVFVYLHGDLLEERQAVREARRAWDERRAALEAQRAAAREAEERRRREGERDR
ATEQEQGPGVRHPDKPRWQVERELILTEVYGSAVAEHMGRWYRIERRADALVLSNREASV
TDFGDRVVAHEGNDKEIEALVLLAQAKGWERVTLTGPREFQERAAQAFVKAGIALEDQAL
EARAKEAIERQRREDIRERALLRQGYRRDAWEIRERWHWRQQERARRLEAERELARIEEE
AEQARDAWRTVWRKTGHLVDADAQEPIPWGIWREQTLAERYGPDIAARAEAEYWYTRLRP
DLGGLDISRNGQEVVDAGAALQVCSAGSMSLAVDLVRAKGWKRVAIGGPEDFRERAARAL
IEAGMVLADEALEQRAKAALDAERVQQERERALRRAMEGIKAHRIRWRRKDGTMGEQRAL
DYLEDRLFRGFTIQETAEGFRWWSADGKPGAVVGDAQLMALFRAAKAADQDLVRVVERIR
MEVGMPNAAADRARGRTVTPKRGLGDDW
Reference
[1] van Zyl LJ et al (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]


II. Auxiliary DNA-binding protein(s) of the relaxosome
# ID Name GenBank Length Note
1 70 _ insolico _ _ _
 N/A
Information of plasmid
ID 42
Plasmid name pTcM1
GenBank accession number NC_010600.1
Genome size 65158 bp
Coordinate of oriT  [Strand]
Drug resistance _
Heavy-metal resistance insolico arsenic resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Acidithiobacillus caldus MNG [33059]
Reference
[1] van Zyl LJ et al (2008) Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus. Appl Environ Microbiol. 74(14):4300-8. [PMID:18515486]

Microbial Bioinformatics Group in MML, SJTU