 oriTDB accession number | 100041 |
| Name | oriT_pBS228  |
Sequence Completeness  | intact |
| oriT length | 112 nt |
| oriT sequence | CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAG TGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG |
| IR (inverted repeat) | [60-78] [81-99] nt (GTGAAGAAGGAACACCCGC.. GCGGGTGGGCCTACTTCAC) |
| Location of nic site | [107-108] nt |
| Conserved sequence flanking the nic site | TATCCTG|C |
| Note | identical to the oriT of RK2 |
Visualization of oriT structure Reference| [1] Haines AS et al (2007) Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny. Plasmid. 58(1):76-83. [PMID:17320955] |
| ID | 39 |
| Name | TraI _pBS228 |
| GenBank accession number | YP_758719 |
| Family | MOBP |
| Length | 732 aa |
| UniProt ID | Q0E689 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 7.90E-67, Aligned region: 12..252 |
| Note | conjugal transfer relaxase TraI |
| Protein sequence [Download] | MIAKHVPMRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANTLPAVMAEVMATQHGN TRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQRVSAVHHDTDNLHIHIA INKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQRVSENRANDMERHAGVE SLVGWIKRECLPELQAAQSWEDLHRVLRENGLKLRERGNGFIFEAGDGTTVKASTVSRDL SKPKLEARFGAFTPAEGGEAPRRREYRAKPLKTRIDTTELYARYQSERQEMGAVRKGELD TLRRRRDRLIEAAMRSNRLRRAAIKLLGEGRIAKRLMYAQAHKALRADLDKINREYRQGR QAVQERTQRRAWADWLKAEAMKGDDKALAALRAREGRSDLKGNTIQGSGEAKPGHAAVTD NITKKGTIIYRVGSSAVRDDGDRLQVSREATTDGLDAALRLAMERFGDRITVNGTAEFKE RIAQAAAAGRLAITFDDAALERRRQELLTKEQAHEQPERNDGRRDRGGDGGIRPAAARTT LNATGGDGDRRDARAVSAGGTVALRKPNVGRIGRKPPPQSQNRLRALSQLGVVRIAGGAE MLLPRDVPGHVEQQGAEPAHALRRGVSGPGRGLKPEQIAAAEKYVAEREQKRLNGFDIPK HARYTDYVGALSYAGTRNVEDQALALLRKENDEILVLPVDKATVQRMKRLAIGDPVTVTP RGSLKTTRGRSR |
Reference| [1] Haines AS et al (2007) Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny. Plasmid. 58(1):76-83. [PMID:17320955] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 63 | TraH_pBS228 | YP_758720 | 119 aa | relaxosome stabilisation |
| 2 | 64 | TraJ_pBS228 | YP_758722 | 123 aa | conjugal transfer relaxosome component TraJ |
| 3 | 65 | TraK_pBS228 | YP_758723 | 134 aa | TraK oriT binding protein |
| ID | 39 |
| Name | TraG_pBS228 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_758718 |
| Family | VirD4/TraG |
| Length | 635 |
| UniProt ID | Q0E690 |
| PDB ID | _ |
| Pfam | T4SS-DNA_transf [PF02534.13], Evalue: 2.20E-174, Aligned region: 120..624 TraG-D_C [PF12696.6], Evalue: 2.70E-31, Aligned region: 443..568 TrwB_AAD_bind [PF10412.8], Evalue: 1.80E-23, Aligned region: 175..575 |
| Note | conjugal transfer coupling protein TraG |
| Protein sequence [Download] | MKNRNNAVGPQIRAKKPKASKTVPILAGLSLGAGLQTATQYFAHSFQYQAGLGWNINHVY TPWSILQWAGKWYGQYPDDFMRAASMGMVVSTVGLLGTAVTQMVKANTGKANDYLHGSAR WADKKDIQAAGLLPRPRTVVELVSGKHPPTSSGVYVGGWQDKDGKFHYLRHNGPEHVLTY APTRSGKGVGLVVPTLLSWAHSAVITDLKGELWALTAGWRKKHARNKVVRFEPASAQGSA CWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLESHWQKTSQALLVGVILHALYKAKNE GTPATLPSVDGMLADPNRDVGELWMEMTTYGHVDGQNHPAVGSAARDMMDRPEEESGSVL STAKSYLALYRDPVVARNVSKSDFRIKQLMHHDDPVSLFIVTQPNDKARLRPLVRVMVNM IVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLEILQESLAFVAGYGIKCYLICQD INQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSKLTGTTTIVKEQITTSGRRTS ALLGNVSRTFQEVQRPLLTPDECLRMPGPKKSADGSIEEAGDMVVYVAGYPAIYGKQPLY FKDPIFQARAAVPAPKVSDKLIQTATVEEGEGITI |
Reference| ID | 39 |
| Plasmid name | pBS228 |
| GenBank accession number | NC_008357.1 |
| Incompatibility group | IncP1 |
| Genome size | 89147 bp |
| Coordinate of oriT [Strand] | 80181..80292 [+] |
| Drug resistance |  resistant to tetracycline |
| Heavy-metal resistance | pBS228 contained mercury resistant genes,but not conferred resistance to mercury. |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Pseudomonas aeruginosa ML4262 [287] |
Reference| [1] Haines AS et al (2007) Sequence of plasmid pBS228 and reconstruction of the IncP-1alpha phylogeny. Plasmid. 58(1):76-83. [PMID:17320955] |