oriTDB

The information of the oriT region

oriTDB accession number	100040
Name	oriT_BRA100
Sequence Completeness	intact
oriT length	373 nt
oriT sequence	GCCTCGCCTCGTCTCTCATGCCGGCGGTAGCCGGCTGCCTCGCAGAGCAGGATGACCCGT TGAGCGCCCCCGGCGCGAATAAGGGACAGTGAAGATAGATAACCGGCTCGCCGGTTAGCT AACTTCACACATCCTGCCCGCCTTACGGCGTTAATAACACCAAGGAAAGTCTACACCAGC CATTACGATTTATCCGCAACTATCGCGCTATCAGGCCGCAAAAGCAGCAACGGATATAGC GAAAACCGCCACAATGGCCCATAATGCCGCTATCGAAGCGTGCCAATGCACGCCGATAGC GGACTTTTTGCGTTTCCGTAGCGCCGCTTAGTAGCGTTACATTTGCGATGAGAGGATTAG ATGGACGAACACG
IR (inverted repeat)	[89-107] [110-128] nt (GTGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCAC)
Location of nic site	[136-137]nt
Conserved sequence flanking the nic site	CATCCTG\|C
Note	_
	L:89-107;R:110-128;D:136-137

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	38
Name	TraI_BRA100
GenBank accession number	AFJ38144
Family	MOB_P
Length	746 aa
UniProt ID	I2ALH0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.90E-64, Aligned region: 12..252
Note	relaxase
Protein sequence [Download]	MIAKHVPMRSLGKSDFAGLANYITDAQSKDHRLGHVQATNCEAGSIQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICVGLGYGEHQRISAVHNDTDNLHIHIA INKIHPTRHTMHEPYYPHRALAELCTALERDYGLERDNHEPRKRGAEGRAADMERHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMRDNGMELRVRANGLVFEAGDGTMVKASTVARDL SKPSLEARLGPFEASPERQAQTTAKRQYRKDPIRLRVNTVELYAKYKAEQQSLTTARAQA LERARHRKDRLIEAAKRSNRLRRATIKVVGEGRANKKLLYAQASKALRSELQAINKQFQQ ERTALYAEHSRRTWADWLKKEAQHGGADALAALRAREAAQGLKGNTIRGEGQAKPGHAPA VDNITKKGTIIFRAGMSAVRDDGDRLQVSREATREGLQEALRLAMQRYGNRITVNGTVEF KAQMIRAAVDSQLPITFTDPALESRRQALLNKENTHERTERPEHRGRTGRGAGGPGQRPA ADQHATGAAAVARAGDGRPAAGRGDRADAGLHAATVHRKPDVGRLGRKPPPQSQHRLRAL SELGVVRIAGGSEVLLPRDVPRHVEQQGTQPDHALRRGISRPGTGVGQTPPGVAAADKYI AEREAKRLKGFDIPKHSRYTAGDGALTFQGTRTIEGQALALLKRGDEVMVMPIDQATARR LTRIAVGDAVSITAKGSIKTSKGRSR

Reference

[1] Leão SC et al (2013) The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. Bolletii. PLoS One. 8(4):e60746. [PMID:23565273]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	60	TraH_BRA100	AFJ38145	130 aa	relaxosome stabilisation protein involved in conjugative plasmid transfer
2	61	TraJ_BRA100	AFJ38146	124 aa	conjugal transfer relaxosome component involved in conjugative plasmid transfer
3	62	TraK_BRA100	AFJ38147	132 aa	binds oriT during conjugative plasmid transfer

Information of T4CP

ID	38
Name	TraG_BRA100
SecReT4 accesion number	_
GenBank accession number	AFJ38143
Family	VirD4/TraG
Length	637
UniProt ID	I2ALG9
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 2.70E-162, Aligned region: 121..624 TraG-D_C [PF12696.6], Evalue: 5.50E-32, Aligned region: 444..569 TrwB_AAD_bind [PF10412.8], Evalue: 5.10E-22, Aligned region: 187..586
Note	DNA transport during conjugative transfer
Protein sequence [Download]	MKIKMNNAVGPQVRTAKPKPSKLLPVLGAASMVGGLQAATQFFAHTFAYHATLGPNVGHV YAPWSILHWTYKWYSQYPDEIMKAGSMGMLVSTVGLLGVAVAKVVTSNSSKANEYLHGSA RWAEKKDIQAAGLLPRERNVLEIVTGKAAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASTSGG VCWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKAKD DGGTATLPSVDAMLADPNRDIGELWMEMATYGHVDGQNHHAIGSAARDMMDRPEEEAGSV LSTAKSYLALYRDPVVARNVSRSDFRIKQLMHEDDPVSLYIVTQPNDKARLRPLVRVMVN MIVRLLADKMDFEGGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ DINQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNAQGEIEEAGDMVIYVAGYPAIYGKQPL YFKDPVFSARAAIPAPKVSDRLRAVAQAETEGEGITI

Reference
N/A

Information of plasmid

ID	38
Plasmid name	BRA100
GenBank accession number	CP003505.2
Incompatibility group	IncP1
Genome size	56265 bp
Coordinate of oriT [Strand]	31224..31596 [-]
Drug resistance	resistance to kanamycin and tobramycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Mycobacterium abscessus subsp. bolletii F1725 [1168066]

Reference

[1] Leão SC et al (2013) The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. Bolletii. PLoS One. 8(4):e60746. [PMID:23565273]