oriTDB

The information of the oriT region

oriTDB accession number	100039
Name	oriT_pTB11
Sequence Completeness	intact
oriT length	100 nt
oriT sequence	GAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTG CCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACAC
IR (inverted repeat)	[13-31] [34-52] nt (GTGAAGAAGGAACACCCGC..GCGGGTGGGCCTACTTCAC)
Location of nic site	[60-61]nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	_
	L:13-31;R:34-52;D:60-61

Visualization of oriT structure

Reference

[1] Tennstedt T et al (2005) Sequence of the 68,869 bp IncP-1alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. Plasmid. 53(3):218-38. [PMID:15848226]

I. Information of Relaxase

ID	37
Name	TraI_pTB11
GenBank accession number	CAG30901
Family	MOB_P
Length	732 aa
UniProt ID	Q5ZHE0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.90E-67, Aligned region: 12..252
Note	relaxase
Protein sequence [Download]	MIAKHVPMRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANTLPAVMAEVMATQHGN TRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQRVSAVHHDTDNLHIHIA INKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQRVSENRANDMERHAGVE SLVGWIKRECLPELQAAQSWEDLHRVLRENGLKLRERGNGFIFEAGDGTTVKASTVSRDL SKPKLEARFGAFTPAEGGEAPRRREYRAKPLKTRIDTTELYARYQSERQEMGAVRKGELD TLRRRRDRLIEAAMRSNRLRRAAIKLLGEGRIAKRLMYAQAHKALRADLDKINREYRQGR QAVQERTQRRAWADWLKAEAMKGDDKALAALRAREGRSDLKGNTIQGSGEAKPGHAAVTD NITKKGTIIYRVGSSAVRDDGDRLQVSREATTDGLDAALRLAMERFGDRITVNGTAEFKE RIAQAAAAGRLAITFDDAALERRRQELLTKEQAHEQPERNDGRRDRGGDGGIRPAAARTT LNATGGDGDRRDARAVSAGGTVALRKPNVGRIGRKPPPQSQNRLRALSQLGVVRIAGGAE MLLPRDVPGHVEQQGAEPAHALRRGVSGPGRGLKPEQIAAAEKYVAEREQKRLNGFDIPK HARYTDYVGALSYAGTRNVEDQALALLRKENDEILVLPVDKATVQRMKRLAIGDPVTVTP RGSLKTTRGRSR

Reference

[1] Tennstedt T et al (2005) Sequence of the 68,869 bp IncP-1alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. Plasmid. 53(3):218-38. [PMID:15848226]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	57	TraH_pTB11	CAG30902	119 aa	involved in conjugative DNA transfer; relaxosome stabilisation
2	58	TraJ_pTB11	CAG30903	123 aa	oriT-binding protein
3	59	TraK_pTB11	CAG30904	134 aa	involved in conjugative DNA transfer

Information of T4CP

ID	37
Name	TraG_pTB11
SecReT4 accesion number	53793956
GenBank accession number	CAG30900
Family	VirD4/TraG
Length	635
UniProt ID	Q5ZHE1
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 2.20E-174, Aligned region: 120..624 TraG-D_C [PF12696.6], Evalue: 2.70E-31, Aligned region: 443..568 TrwB_AAD_bind [PF10412.8], Evalue: 1.80E-23, Aligned region: 175..575
Note	conjugal transfer coupling protein TraG; Provisional
Protein sequence [Download]	MKNRNNAVGPQIRAKKPKASKTVPILAGLSLGAGLQTATQYFAHSFQYQAGLGWNINHVY TPWSILQWAGKWYGQYPDDFMRAASMGMVVSTVGLLGTAVTQMVKANTGKANDYLHGSAR WADKKDIQAAGLLPRPRTVVELVSGKHPPTSSGVYVGGWQDKDGKFHYLRHNGPEHVLTY APTRSGKGVGLVVPTLLSWAHSAVITDLKGELWALTAGWRKKHARNKVVRFEPASAQGSA CWNPLDEIRLGTEYEVGDVQNLATLIVDPDGKGLESHWQKTSQALLVGVILHALYKAKNE GTPATLPSVDGMLADPNRDVGELWMEMTTYGHVDGQNHPAVGSAARDMMDRPEEESGSVL STAKSYLALYRDPVVARNVSKSDFRIKQLMHHDDPVSLFIVTQPNDKARLRPLVRVMVNM IVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLEILQESLAFVAGYGIKCYLICQD INQLKSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSKLTGTTTIVKEQITTSGRRTS ALLGNVSRTFQEVQRPLLTPDECLRMPGPKKSADGSIEEAGDMVVYVAGYPAIYGKQPLY FKDPIFQARAAVPAPKVSDKLIQTATVEEGEGITI

Reference

[1] Tennstedt T et al (2005) Sequence of the 68,869 bp IncP-1alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. Plasmid. 53(3):218-38. [PMID:15848226]

Information of plasmid

ID	37
Plasmid name	pTB11
GenBank accession number	AJ744860.1
Incompatibility group	IncP1
Genome size	68869 bp
Coordinate of oriT [Strand]	55435..55534 [+]
Drug resistance	Resistance spectrum: ampicillin, cefaclor, cefuroxime, gentamicin, kanamycin, streptomycin, spectinomycin, tetracycline and tobramycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Tennstedt T et al (2005) Sequence of the 68,869 bp IncP-1alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements. Plasmid. 53(3):218-38. [PMID:15848226]