oriTDB

The information of the oriT region

oriTDB accession number	100037
Name	oriTA_pSKU146
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	TATGAACTTCCCCACCAAGATATTTTGATAAAATGACTTGTTGCACAAAATGACATTGAG CACTACCAAACTGAACGGGAGTTAAAAATGGAAAATGGTAATCTTAAGGGATATCCTGAC TTAATTATTTACAAAAATGATGATAGTGAAATTTATATAGAATTTGAACGAACAC
IR (inverted repeat)	IR1: [16-26] [30-40] nt (CAAGATATTTT..AAAATGACTTG) IR2: [42-48] [56-62] nt (TGCACAA..TTGAGCA) IR3: [107-112] [113-117] nt (AGGGAT..ATCCT) IR4: [124-130] [135-141] nt (ATTATTT..AAATGAT) IR5: [146-153] [161-169] nt (GTGAAATT..AATTTGAAC)
Location of nic site	[118-119] nt
Conserved sequence flanking the nic site	TATCCTG\|A
Note	putative oriT region; oriTA
	L:16-26;R:30-40;L:42-48;R:56-62;L:107-112;R:113-117;L:124-130;R:135-141;L:146-153;R: 161-169;D: 118-119

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TATGAACTTCCCCACCAAGATATTTTGATAAAATGACTTGTTGCACAAAATGACATTGAG

61

CACTACCAAACTGAACGGGAGTTAAAAATGGAAAATGGTAATCTTAAGGGATATCCTGAC

121

TTAATTATTTACAAAAATGATGATAGTGAAATTTATATAGAATTTGAACGAACAC

Reference

[1] Davis RE et al (2005) Cryptic plasmid pSKU146 from the wall-less plant pathogen Spiroplasma kunkelii encodes an adhesin and components of a type IV translocation-related conjugation system. Plasmid. 53(2):179-90. [PMID:15737404]

N/A

Information of T4CP

ID	36
Name	Mob_pSKU146
SecReT4 accesion number	_
GenBank accession number	YP_138235
Family	TrwB/TraD
Length	504
UniProt ID	Q5VCB6
PDB ID	_
Pfam	TrwB_AAD_bind [PF10412.8], Evalue: 4.10E-06, Aligned region: 122..183 TrwB_AAD_bind [PF10412.8], Evalue: 2.00E-09, Aligned region: 265..491 TraG-D_C [PF12696.6], Evalue: 6.10E-11, Aligned region: 351..468 DUF87 [PF01935.16], Evalue: 5.80E-06, Aligned region: 123..357
Note	contains VirD4 domain; similar to Mob protein of Streptococcus mutans accession number NP_114056, conjugative coupling factor of Vibrio cholerae GenBank Accession Number AAL59680
Protein sequence [Download]	MLKKIINWRDKNISNKIIYWFIFIFVVPFILLCFINCASAIIIMFLKYHTLDFKNFHIAY VDKYSWIISLVLFVIGFLFFLWFYVCHKIDNMDSPKIKQQKSSKASDKEFKTLQLKMLAL NNKYGIPKTNLSQHTILVGTTGSGKTTTLMFLVKQLTQLFKQTTIIIDGKGDIDLINKVK QQDPNAFIWEIGGTTEYNPFATKDSVVLSDKIMSLFTFSEPHYEALVNDYVLILTETLIN KNVELTLENIIKYFDIKELKQLISKKDNNYEYLEKLNEDDILGMRSRLNVYRQQLKTNIG INNKLLELITKHKTILFSINSLMYPKLAGSVGKIIIQDLKELTTLKPVNQKINIVLDEFN VFASETIINLINKSRSFNYQCFLSFQTINDLKTNNMNLTDTIFGNVNSIVCHNIKDPNTT EYVASVFGTQETEKLTRQLDFKNNTADMGSVRSVDEFVVHPNDLKTLKIGECYFKTTLPS GKLFIKKIKINPTYLDGVFQKLNK

Reference

[1] Davis RE et al (2005) Cryptic plasmid pSKU146 from the wall-less plant pathogen Spiroplasma kunkelii encodes an adhesin and components of a type IV translocation-related conjugation system. Plasmid. 53(2):179-90. [PMID:15737404]

Information of plasmid

ID	36
Plasmid name	pSKU146
GenBank accession number	NC_006400.1
Incompatibility group	IncP1
Genome size	14615 bp
Coordinate of oriT [Strand]	377..551 [+]; 1160..1334 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	Plasmid pSKU146 harbors genes encoding putative proteins similar to known bacterial virulence factors and conjugative elements including Mob family proteins, origins of plasmid transfer, Vir domains, ICEF ORF-like proteins,and the ScARP1 adhesin of S. citri, implicating a role of pSKU146-encoded genes in conjugal cell-to-cell ommunication and pathogenesis.
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Spiroplasma kunkelii CR2-3x [273035]

Reference

[1] Davis RE et al (2005) Cryptic plasmid pSKU146 from the wall-less plant pathogen Spiroplasma kunkelii encodes an adhesin and components of a type IV translocation-related conjugation system. Plasmid. 53(2):179-90. [PMID:15737404]