 oriTDB accession number | 100034 |
| Name | oriT_pV404  |
Sequence Completeness  | intact |
| oriT length | 83 nt |
| oriT sequence | CGGGACAGGATGTGTAATTGTAATACCGTCACACGCGACGCTATTACAATTACCATCTGG TCAGGGCTTCGCCCCGACACCCC |
| IR (inverted repeat) | 8-bp: [6-13] [17-24] nt (GTCGGGGC..GCCCTGAC) 17-bp: [31-47] [54-70] nt (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC) |
| Location of nic site | [78-79] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | minimal oriT sequence |
Visualization of oriT structure Reference| [1] Riccobono E et al (2014) Complete sequence of pV404, a novel IncI1 plasmid harbouring blaCTX-M-14 in an original genetic context. Int J Antimicrob Agents. 44(4):374-6. [PMID:25178920] |
| ID | 33 |
| Name | NikB_pV404 |
| GenBank accession number | CDW92102 |
| Family | MOBP |
| Length | 899 aa |
| UniProt ID | A0A078BC53 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.90E-62, Aligned region: 48..315 |
| Note | NikB relaxase |
| Protein sequence [Download] | MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRNRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHY ILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYL NCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMNGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVVIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG |
Reference| [1] Riccobono E et al (2014) Complete sequence of pV404, a novel IncI1 plasmid harbouring blaCTX-M-14 in an original genetic context. Int J Antimicrob Agents. 44(4):374-6. [PMID:25178920] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 50 | NikA_pV404 | CDW92101 | 110 aa | NikA oriT-specific DNA binding protein |
| ID | 33 |
| Name | TrbC_pV404 |
| SecReT4 accesion number | _ |
| GenBank accession number | CDW92107 |
| Family | VirD4/TraG |
| Length | 763 |
| UniProt ID | A0A078BC56 |
| PDB ID | _ |
| Pfam | |
| Note | TrbC transfer protein |
| Protein sequence [Download] | MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISV DAPRLDRLRRLVPRIAQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQI SVRTERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY |
Reference| ID | 33 |
| Plasmid name | pV404 |
| GenBank accession number | LM651376.1 |
| Incompatibility group | IncI1 |
| Genome size | 101631 bp |
| Coordinate of oriT [Strand] | 42424..42506 [-] |
| Drug resistance | beta-lactamase gene blaCTX-M-14 |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli V404 [562] |
Reference| [1] Riccobono E et al (2014) Complete sequence of pV404, a novel IncI1 plasmid harbouring blaCTX-M-14 in an original genetic context. Int J Antimicrob Agents. 44(4):374-6. [PMID:25178920] |