 oriTDB accession number | 100033 |
| Name | oriT_pSTM709  |
Sequence Completeness  | intact |
| oriT length | 88 nt |
| oriT sequence | TGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTAC AATTACACATCCTGTCCCGTTTTTCAGG |
| IR (inverted repeat) | 8-bp: [2-9] [13-20] nt (GTCGGGGC..GCCCTGAC) 17-bp: [27-43] [50-66] nt (GTAATTGTAATAGCGTC..GACGGTATTACAATTAC) |
| Location of nic site | [74-75] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | _ |
Visualization of oriT structure Reference| ID | 32 |
| Name | NikB_pSTM709 |
| GenBank accession number | YP_009023753 |
| Family | MOBP |
| Length | 899 aa |
| UniProt ID | X5JA84 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.40E-62, Aligned region: 47..315 |
| Note | NikB relaxase |
| Protein sequence [Download] | MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELNLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHY ILSWQAHESPRPEQIYDSVRHTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYL NCLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG |
Reference| # | ID | Name | GenBank | Length | Note |
| 1 | 49 | NikA_pSTM709 | YP_009023752 | 110 aa | NikA oriT-specific DNA binding protein |
| ID | 32 |
| Name | TrbC_pSTM709 |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_009023754 |
| Family | VirD4/TraG |
| Length | 763 |
| UniProt ID | X5JAP4 |
| PDB ID | _ |
| Pfam | |
| Note | TrbC transfer protein |
| Protein sequence [Download] | MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKIDVQELIELHPGEFFSIFRGETVPSASFFIPDNEKSCSSDPVVINRYISV DAPRLDRLRRLVPRTAQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQI SVRAERAGINLLTLPQDNNHLTGRPVNGFHHKKTNRPDWDGMY |
Reference| ID | 32 |
| Plasmid name | pSTM709 |
| GenBank accession number | NC_023915.1 |
| Incompatibility group | IncI1 |
| Genome size | 99184 bp |
| Coordinate of oriT [Strand] | 43906..43949 [-] |
| Drug resistance | class-C beta-lactamase gene blaCMY-2 |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica serovar Typhimurium str. STM709 [1401642] |
Reference| [1] Phan MD et al (2015) Third-generation cephalosporin resistance conferred by a chromosomally encoded blaCMY-23 gene in the Escherichia coli ST131 reference strain EC958. J Antimicrob Chemother. 70(7):1969-72. [PMID:25786480] |