oriTDB

The information of the oriT region

oriTDB accession number	100032
Name	oriT_pEK204
Sequence Completeness	intact
oriT length	102 nt
oriT sequence	ACTCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAA TAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCC
IR (inverted repeat)	8-bp: [26-33] [37-44] nt (GTCGGGGC..GCCCTGAC) 17-bp: [51-67] [74-90] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC)
Location of nic site	[98-99] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	minimal oriT sequence
	L:26-33;R:37-44;L:51-67;R:74-90;D: 98-99

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACTCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAA

61

TAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCC

Reference

[1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243]

I. Information of Relaxase

ID	31
Name	NikB_pEK204
GenBank accession number	ACQ42157
Family	MOB_P
Length	899 aa
UniProt ID	C7SA36
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.70E-63, Aligned region: 47..315
Note	relaxase
Protein sequence [Download]	MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL AERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

Reference

[1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	48	NikA_pEK204	ACQ42156	110 aa	plasmid mobilization protein

Information of T4CP

ID	31
Name	TrbC_pEK204
SecReT4 accesion number	_
GenBank accession number	ACQ42158
Family	TrwB/TraD
Length	764
UniProt ID	C7SA37
PDB ID	_
Pfam
Note	Type IV Secretion System Coupling Protein
Protein sequence [Download]	MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISV DAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQI SVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

Reference

[1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243]

Information of plasmid

ID	31
Plasmid name	pEK204
GenBank accession number	EU935740.1
Incompatibility group	IncI1
Genome size	93732 bp
Coordinate of oriT [Strand]	36439..36540 [-]
Drug resistance	beta-lactamases: CTX-M-3 enzyme and TEM-1 enzyme (resistance genes: bla_CTX-M-3 and bla_TEM-1)
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli C [562]

Reference

[1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243]