 oriTDB accession number | 100032 |
| Name | oriT_pEK204  |
Sequence Completeness  | intact |
| oriT length | 102 nt |
| oriT sequence | ACTCACTTCAGGCTCCTTACGGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAA TAGCGTCGCGTGTGACGGTATTACAATTGCACATCCTGTCCC |
| IR (inverted repeat) | 8-bp: [26-33] [37-44] nt (GTCGGGGC..GCCCTGAC) 17-bp: [51-67] [74-90] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | [98-99] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | minimal oriT sequence |
Visualization of oriT structure Reference| [1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243] |
| ID | 31 |
| Name | NikB_pEK204 |
| GenBank accession number | ACQ42157 |
| Family | MOBP |
| Length | 899 aa |
| UniProt ID | C7SA36 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 2.70E-63, Aligned region: 47..315 |
| Note | relaxase |
| Protein sequence [Download] | MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSHMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL AERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALVDKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG |
Reference| [1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 48 | NikA_pEK204 | ACQ42156 | 110 aa | plasmid mobilization protein |
| ID | 31 |
| Name | TrbC_pEK204 |
| SecReT4 accesion number | _ |
| GenBank accession number | ACQ42158 |
| Family | TrwB/TraD |
| Length | 764 |
| UniProt ID | C7SA37 |
| PDB ID | _ |
| Pfam | |
| Note | Type IV Secretion System Coupling Protein |
| Protein sequence [Download] | MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISV DAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERRIRYITLNRPELPETKEENQI SVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY |
Reference| [1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243] |
| ID | 31 |
| Plasmid name | pEK204 |
| GenBank accession number | EU935740.1 |
| Incompatibility group | IncI1 |
| Genome size | 93732 bp |
| Coordinate of oriT [Strand] | 36439..36540 [-] |
| Drug resistance |  beta-lactamases: CTX-M-3 enzyme and TEM-1 enzyme (resistance genes: blaCTX-M-3 and blaTEM-1) |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli C [562] |
Reference| [1] Woodford N et al (2009) Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob Agents Chemother. 53(10):4472-82. [PMID:19687243] |