 oriTDB accession number | 100028 |
| Name | oriT_R621a  |
Sequence Completeness  | intact |
| oriT length | 83 nt |
| oriT sequence | GGGGTGTCGGGGCGAAGCCCTGACCAGATGGCAATTGTAATAGCGTCGCGTGTGACGGTA TTACAATTGCACATCCTGTCCCG |
| IR (inverted repeat) | 8-bp: [6-13] [17-24] nt (GTCGGGGC..GCCCTGAC) 17-bp: [31-7][54-70] nt (GCAATTGTAATAGCGTC..GACGGTATTACAATTGC) |
| Location of nic site | [78-79] nt |
| Conserved sequence flanking the nic site | CATCCTG|T |
| Note | minimal oriT sequence |
Visualization of oriT structure Reference| [1] Takahashi H et al (2011) The genome sequence of the incompatibility group Iγ plasmid R621a: evolution of IncI plasmids. Plasmid. 66(2):112-21. [PMID:21763721] |
| ID | 27 |
| Name | NikB_R621a |
| GenBank accession number | YP_004823733 |
| Family | MOBP |
| Length | 899 aa |
| UniProt ID | G2HK77 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 7.60E-64, Aligned region: 47..315 |
| Note | NikB relaxase |
| Protein sequence [Download] | MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWLPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG |
Reference| [1] Takahashi H et al (2011) The genome sequence of the incompatibility group Iγ plasmid R621a: evolution of IncI plasmids. Plasmid. 66(2):112-21. [PMID:21763721] |
| # | ID | Name | GenBank | Length | Note |
| 1 | 41 | NikA_R621a | YP_004823732 | 111 aa | NikA oriT-specific DNA-binding protein |
| ID | 27 |
| Name | TrbC_R621a |
| SecReT4 accesion number | _ |
| GenBank accession number | YP_004823734 |
| Family | TrwB/TraD |
| Length | 763 |
| UniProt ID | G2HK78 |
| PDB ID | _ |
| Pfam | |
| Note | TrbC transfer protein |
| Protein sequence [Download] | MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTMITML VFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQR VRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTAR TIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTRPQSNTWNPFCYSTEAFTAETMQ SMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSLQMLREHMTLEGMAKLYCRGL DDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSETFSTFTEAFG DIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILTAQDQERIEGQ TTATNTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDS PQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISV DAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIA GRQAAMAMLEEYDTDINARESALWETAVNTLNTTTREERRIRYITLNRPELPETKEENQI SVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY |
Reference| [1] Takahashi H et al (2011) The genome sequence of the incompatibility group Iγ plasmid R621a: evolution of IncI plasmids. Plasmid. 66(2):112-21. [PMID:21763721] |
| ID | 27 |
| Plasmid name | R621a |
| GenBank accession number | NC_015965.1 |
| Incompatibility group | IncI |
| Genome size | 93185 bp |
| Coordinate of oriT [Strand] | 37332..37414 [-] |
| Drug resistance |  resistance to tetracycline |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Salmonella enterica serovar Typhimurium [90371] |
Reference| [1] Takahashi H et al (2011) The genome sequence of the incompatibility group Iγ plasmid R621a: evolution of IncI plasmids. Plasmid. 66(2):112-21. [PMID:21763721] |