oriTDB

The information of the oriT region

oriTDB accession number	100027
Name	oriT_pB4
Sequence Completeness	core
oriT length	92 nt
oriT sequence	GAATAAGGGACAGCGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCGCCTATCCTG CCCGCCTTACGACGTCTTTTACTCCAAGAATG
IR (inverted repeat)	[13-31] [34-52] nt (GCGAAGATAGATAACCGGC..GCCGGTTAGCTAACTTCGC)
Location of nic site	[60-61] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	_
	L:13-31;R:34-52;D:60-61

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GAATAAGGGACAGCGAAGATAGATAACCGGCTTGCCGGTTAGCTAACTTCGCCTATCCTG

61

CCCGCCTTACGACGTCTTTTACTCCAAGAATG

Reference
N/A

I. Information of Relaxase

ID	26
Name	TraI_pB4
GenBank accession number	CAD24395
Family	MOB_P
Length	752 aa
UniProt ID	Q8RSH1
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 4.30E-61, Aligned region: 12..252
Note	relaxase
Protein sequence [Download]	MIVKHVPMRSLGKSDFAGLVNYVTDAQSKDHRLGQVQITNCDAMSVQDAITEVLATQHTN TRAKGDKTYHLIVSFRAGEQPSADTLRAIEERICAGLGFAEHQRVSAVHNDTDNLHIHIA INKIHPTRHTMHEPFGQHRTLAELCVALERDYGLERDNHEPRQRGAEGRAADMERHAGVE SLVGWIKRECLDEIKGAQSWQELHQVMHDNGLELRERANGLVIEAGDGTMVKASTLARDL SKPKLEARLGPFEASPERQTRTKAKRQYQKDPIRLRVNTVELYARYKAEQQTLTATRGQA LEQARRRKDRLVEAAKRSNRLRRATIKMVGENRTNKKLLYAQASKALRDEIQAINKQYQQ DRQALYDSHSRRTWADWLKKEATHGNAEALAALRAREAAQGLQGNTIQGRGDARPGHAPV TDNITKKGTIIFRAGLSAVRDDGDKLQVSREATREGLQEALRLAMERYGSRITVNGTTQF KAQVIRAAVDSQLPITFADPALESRRLALLKKENTHERPDRTERADQRPAEHRGRTGRGP GGPGQRAAAHDDAARPVAVARAGFGRPGAGRTDAAGVHAGAVHWKPDIGRIGRVPPPQSQ HRLRTLSQLGVVRIAGGSEVLLPRDVPRHLEQQGTQPDHQLRRGISRPGTGVAAAPPGLA AADKYIAEREAKRSKGFDIPKHARYTAGDGALAFQGVRNIEGQALALLKRGDEVMVMPID QATARRLSRIAVGDAVTVTPKGSIKTSKGRSR

Reference

[1] Tauch A et al (2003) The 79,370-bp conjugative plasmid pB4 consists of an IncP-1beta backbone loaded with a chromate resistance transposon, the strA-strB streptomycin resistance gene pair, the oxacillinase gene bla(NPS-1), and a tripartite antibiotic efflux system of the resistance-nodulation-division family. Mol Genet Genomics. 268(5):570-84. [PMID:12589432]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	38	TraH_pB4	CAD24396	136 aa	_
2	39	TraJ_pB4	CAD24398	123 aa	_
3	40	TraK_pB4	CAD24399	137 aa	_

Information of T4CP

ID	26
Name	TraG_pB4
SecReT4 accesion number	19352411
GenBank accession number	CAD24394
Family	VirD4/TraG
Length	634
UniProt ID	Q8RSH2
PDB ID	_
Pfam	T4SS-DNA_transf [PF02534.13], Evalue: 1.90E-163, Aligned region: 121..624 TraG-D_C [PF12696.6], Evalue: 7.60E-32, Aligned region: 444..569 TrwB_AAD_bind [PF10412.8], Evalue: 4.50E-22, Aligned region: 177..576
Note	Type IV Secretion System Coupling Protein
Protein sequence [Download]	MKIKMNNSVGPQVRSAKPKTPKLLPILGGVSLVGGLQAATQFFAYTFQYHASLGANLGHV YAPWSILNWSAKWYSQYPDEIMRAGSIGMVTATVGLLGVAVAKVVTSNSSKANEYLHGSA RWAEKKDIQAAGLLPRERNVLEIVTGKDAPTATGVYVGGWQDKDGNFFYLRHSGPEHVLT YAPTRSGKGVGLVVPTLLSWGASSVITDLKGELWALTAGWRQKHAKNKVLRFEPASSSGG VCWNPLDEIRVGTEAEVGDVQNLATLIVDPDGKGLDSHWQKTAFALLVGVILHALYKARN EGGTATLPSVDAMLADPNRDIGELWMEMTTYGHVDGQNHHAIGSAARDMMDRPEEEAGSV LSTAKSYLALYRDPVVARNVSRSDFRIKDLMHSDDPVSLYIVTQPNDKARLRPLVRVMVN MIVRLLADKMDFENGRPVAHYKHRLLMMLDEFPSLGKLEIMQESLAFVAGYGIKCYLICQ DINQLRSRETGYGHDESITSNCHVQNAYPPNRVETAEHLSRLTGQTTVVKEQITTSGRRT AAMLGQVSRTYQEVQRPLLTPDECLRMPGPKKNDKGEIEEAGDMVIYVAGYPAIYGKQPL YFKDPVFQARASIPAPKATDRLRQVVEAGEGITI

Reference

[1] Tauch A et al (2003) . Mol Genet Genomics. 268(5):570-84. [PMID:12589432]

Information of plasmid

ID	26
Plasmid name	pB4
GenBank accession number	AJ431260.1
Incompatibility group	IncP1
Genome size	79370 bp
Coordinate of oriT [Strand]	72051..72142 [+]
Drug resistance	resistance to streptomycin, erythromycin and amoxicillin The plasmid contains the the beta-lactam antibiotic resistance gene bla_NPS-1.
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Uncultured bacterium [77133]

Reference

[1] Tauch A et al (2003) The 79,370-bp conjugative plasmid pB4 consists of an IncP-1beta backbone loaded with a chromate resistance transposon, the strA-strB streptomycin resistance gene pair, the oxacillinase gene bla(NPS-1), and a tripartite antibiotic efflux system of the resistance-nodulation-division family. Mol Genet Genomics. 268(5):570-84. [PMID:12589432]