oriTDB

The information of the oriT region

oriTDB accession number	100026
Name	oriT_pK214
Sequence Completeness	incomplete
oriT length	10 nt
oriT sequence	TAGTGTGTTA
IR (inverted repeat)	_
Location of nic site	[7-8] nt
Conserved sequence flanking the nic site	TAGTGTG\|TTA
Note	from GenBank
	D:7-8

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TAGTGTGTTA

Reference

[1] Hickey RM et al (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207]

I. Information of Relaxase

ID	25
Name	Mob_pK214
GenBank accession number	CAA63521
Family	MOB_V
Length	403 aa
UniProt ID	O32791
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 5.10E-77, Aligned region: 1..193
Note	relaxase
Protein sequence [Download]	MSFAVARMQKVKSGNLVGVGNHNQRNTDNHSNKDIDVERSHLNYDLVNRTENYKRDIEQF INDNKSSSRAVRKDAVLINEWIITSDNPFFKDKDDKEIKDFFDTAKSYFADKFGDNNIRY AQVHLDETTPHMHLGIVPFNDEHKLSAKTVFNRQALQAVQDELPKYLNERGFELERGEKG SERKNLTVPEYKKAKDELKEITTTLEQRKSEVLALSNDKAPTIKKESLDLKDETKTVKVP SGETIGIGKLRHEFMKNEERKTGNVIIPESKLNAVIKSYEELYKANEKLKNYAETDLPKE ITRVKEKYRELAKDYNRLVRKSNQNIETLEELEKENKSLKNEIKGIYKGFNQFMENTLGA TVGQAKKLMNNLMSEVKEFVKGGEFEKIHRNETKTRDRDELSR

Reference

[1] Perreten V et al (1997) Antibiotic resistance spread in food. Nature. 389(6653):801-2. [PMID:9349809]

[2] Hickey RM et al (2001) Exploitation of plasmid pMRC01 to direct transfer of mobilizable plasmids into commercial lactococcal starter strains. Appl Environ Microbiol. 67(6):2853-8. [PMID:11375207]

II. Auxiliary DNA-binding protein(s) of the relaxosome

#	ID	Name	GenBank	Length	Note
1	37	_	_	_	_

N/A

Information of plasmid

ID	25
Plasmid name	pK214
GenBank accession number	NC_009751.1
Genome size	29871 bp
Coordinate of oriT [Strand]	7087..7098 [+]
Drug resistance	resistance to tetracyline, chloramphenicol, and streptomycin
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis K214 [889971]

Reference

[1] Perreten V et al (1997) Antibiotic resistance spread in food. Nature. 389(6653):801-2. [PMID:9349809]